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Recombinant Human Carbonic Anhydrase IX / CA9 (38-414) Protein, His tag, 50 µg  

Recombinant Human Carbonic Anhydrase IX / CA9 (38-414) Protein, His tag, 50 µg

Recombinant Human Carbonic Anhydrase IX / CA9 (38-414) Protein, Gln 38 - Asp 414, expressed from human 293 cells, His Tag (active enzyme, MALS verified)

Synonym: Recombinant human protein, CAIX,CA9,CA-IX,G250,MN,P54/58N,pMW1

More details

CA9-H5226-50

Availability: within 7 days

455,00 €

Background
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes. CAs form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. One of the functions of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. The active site of most carbonic anhydrases contains a zinc ion. There are at least five distinct CA families (α, β, γ, δ and ε). Carbonic anhydrase 9 (CA9 / CAIX) is also known as Membrane antigen MN (MN), Renal cell carcinoma-associated antigen G250, which belongs to the alpha-carbonic anhydrase family. CA9 / CAIX with an optimal activity at pH 6.49. Reversible hydration of carbon dioxide. CA IX participates in pH regulation. CA9 may be involved in the control of cell proliferation and transformation. CA-IX appears to be a novel specific biomarker for a cervical neoplasia.

Source
Recombinant Human Carbonic Anhydrase IX (38-414), His Tag (CA9-H5226) is expressed from human 293 cells (HEK293). It contains AA Gln 38 - Asp 414 (Accession # Q16790-1).
Predicted N-terminus: Gln 38

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 41.7 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 20 mM MES, 100 mM NaCl, pH6.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Investigation of carbonic anhydrase inhibitory effects and cytotoxicities of pyrazole-based hybrids carrying hydrazone and zinc-binding benzenesulfonamide pharmacophores"
Yamali, Sakagami, Satoh et al
Bioorg Chem (2022) 127, 105969
(2) "Novel benzenesulfonamide-bearing pyrazoles and 1,2,4-thiadiazoles as selective carbonic anhydrase inhibitors"
KumCarbonic anhydrase IX stratifies patient prognosis and identifies nodal status in animal models of nasopharyngeal carcinoma using a targeted imaging strategy"
Huang, Wang, Huang et al
Eur J Nucl Med Mol Imaging (2022)
(3) "Expression and prognostic value of carbonic anhydrase IX (CA-IX) in bladder urothelial carcinoma"
Xiang, Chen, Xu et al
BMC Urol (2022) 22 (1), 120
Showing 1-3 of 2470 papers.