Recombinant Human CD94 Protein, His Tag, 100 µg

Background
CD94(Natural killer cells antigen CD94) is also known as KLRD1, KP43, NK cell receptor, Killer cell lectin-like receptor subfamily D member 1. CD94 plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. CD94 pairs with the NKG2 molecule as a heterodimer. The CD94/NKG2 complex, on the surface of natural killer cells interacts with Human Leukocyte Antigen (HLA)-E on target cells.

Source
Recombinant Human CD94, His Tag (CD4-H52H8) is expressed from human 293 cells (HEK293). It contains AA Lys 32 - Ile 179 (Accession # Q13241-1).
Predicted N-terminus: Lys 32

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 19.1 kDa. The protein migrates as 24-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20^°C to -70^°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Unraveling factors responsible for pathogenic differences in Lassa virus strains"
Taniguchi, Saito, Paroha et al
bioRxiv (2024)
(2) "Multifactorial determinants of NK cell repertoire organization: insights into age, sex, KIR genotype, HLA typing, and CMV influence"
Ferron, David, Willem et al
Front Immunol (2024) 15, 1389358
(3) "CD8+ T cell targeting of tumor antigens presented by HLA-E"
Iyer, Verweij, Nair et al
Sci Adv (2024) 10 (19), eadm7515
Showing 1-3 of 1103 papers.