Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Cynomolgus / Rhesus macaque BCMA / TNFRSF17 Protein, Fc Tag, 100 µg  

Recombinant Cynomolgus / Rhesus macaque BCMA / TNFRSF17 Protein, Fc Tag, 100 µg

Recombinant Cynomolgus / Rhesus macaque BCMA / TNFRSF17 Protein, AA Met 1 - Ala 53, expressed from human 293 cells (HEK293), Fc Tag

Synonym: Recombinant, cynomolgus, protein, TNFRSF17, CD269, BCM, BCMA

More details

BCA-C5253-100

Availability: within 7 days

420,00 €

Background
Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) is also known as B-cell maturation protein (BCMA), CD antigen CD269, which is a member of the TNF-receptor superfamily. TNFRSF17 contains one TNFR-Cys repeat. TNFRSF17 is expressed in mature B-cells, but not in T-cells or monocytes. TNFRSF17 is receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. TNFRSF17 promotes B-cell survival and plays a role in the regulation of humoral immunity. TNFRSF17 can activate NF-kappa-B and JNK.

Source
Recombinant Cynomolgus / Rhesus macaque BCMA protein, Fc Tag (BCA-C5253) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 53 (Accession # G7Q0I4-1). In the region Met 1 - Ala 53, the AA sequence of Cynomolgus and Rhesus macaque BCMA are homologus.
Predicted N-terminus: Met 1

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 32.1 kDa. As a result of glycosylation, the protein migrates as 37 kDa and 38-42 kDa under reducing (R) condition, and 60-66 kDa under non-reducing (NR) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity-ELISA
Immobilized Human BAFF, His Tag (Cat. No. BAF-H5248) at 5 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque BCMA, Fc Tag (Cat. No. BCA-C5253) with a linear range of 0.05-2 ng/mL (QC tested).

References

(1)  Hatzoglou A., et al., 2000, J. Immunol. 165:1322-1330.
(2)  Gross J.A., et al., 2000, Nature 404:995-999.
(3)  Yu G., et al., 2000, Nat. Immunol. 1:252-256.