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Recombinant Biotinylated Human Fc gamma RIIB / CD32b, Avi Tag (Avitag™),25 µg  

Recombinant Biotinylated Human Fc gamma RIIB / CD32b, Avi Tag (Avitag™),25 µg

Recombinant Biotinylated Human Fc gamma RIIB / CD32b, expressed from human HEK293 cells, Ala 46 - Pro 217, Avi tag (Avitag™), His-Tag, SPR verified

Synonym
recombinant, human, biotinylated, protein, FCGR2B,CD32b,CD32,FCG2,IGFR2,CDw32

More details

CDB-H82E0-25

Availability: within 7 days

420,00 €

Background
Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. Low affinity immunoglobulin gamma Fc region receptor II-b (FCGR2B) is also known as CD32b, FCG2, IGFR2. CD32B is expressed on B cells and myeloid dendritic cells. Ligation of CD32B on B cells downregulates antibody production and may, in some circumstances, promote apoptosis. Co-ligation of CD32B on dendritic cells inhibits maturation and blocks cell activation. CD32B may also be a target for monoclonal antibody therapy for malignancies.

Source
Recombinant Biotinylated Human Fc gamma RIIB / CD32b (CDB-H82E0) is expressed from human HEK293 cells. It contains AA Ala 46 - Pro 217 (Accession # NP_001002273.1).
Predcited N-terminus: Ala 46

Molecular Characterization
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 23 kDa. The protein migrates as 29-32 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Biotin:Protein Ratio
The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Bioactivity
Immobilized Human FGF R4, His Tag (Cat. No. FG4-H5228) at 5 μg/mL (100 μL/well) can bind Biotinylated Human FGF basic, Tag Free (Cat. No. BFF-H8117) with a linear range of 20-156 ng/mL (QC tested).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4) with Trehalose as protectant.
Contact us for customized product form or formulation.

Reconstitution
Reconstitute at 250 μg/mL in sterile deionized water. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

References

(1) Hamada F., et al., 1993, Proc. Natl. Acad. Sci. U.S.A. 90:6305-6309.
(2) Sarmay G., et al., 1997, Immunol. Lett. 57:159-164.
(3) Laine D., et al., 2005, J. Gen. Virol. 86:1771-1784.