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Recombinant Biotinylated Human Fc gamma RIIB / CD32b, Avi Tag (Avitag™),25 µg  

Recombinant Biotinylated Human Fc gamma RIIB / CD32b, Avi Tag (Avitag™),25 µg

Recombinant Biotinylated Human Fc gamma RIIB/C / CD32b/c Protein, (SPR & BLI verified), Ala 46 - Pro 217, expressed from human 293 cells (HEK293), Avitag™,His Tag,

Synonym: recombinant, human, biotinylated, protein, FCGR2B/C, CD32b/c, FcRII-b/c, Fc-gamma RII-b/c, Fc-gamma-RIIb/c, CD32, FCG2, IGFR2, CDw32

More details

CDB-H82E0-25

Availability: within 7 days

420,00 €

Background
Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. Low affinity immunoglobulin gamma Fc region receptor II-b (FCGR2B) is also known as CD32b, FCG2, IGFR2. CD32B is expressed on B cells and myeloid dendritic cells. Ligation of CD32B on B cells downregulates antibody production and may, in some circumstances, promote apoptosis. Co-ligation of CD32B on dendritic cells inhibits maturation and blocks cell activation. CD32B may also be a target for monoclonal antibody therapy for malignancies.

Source
Recombinant Biotinylated Human CD32b, Avitag,His Tag (CDB-H82E0) is expressed from human 293 cells (HEK293). It contains AA Ala 46 - Pro 217 (Accession # P31994-1).
Predicted N-terminus: Ala 46

Molecular Characterization
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 23.0 kDa. The protein migrates as 25 kDa and 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Biotin:Protein Ratio
The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

References

Please refer to product data sheet.