Products

Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Biotinylated Human IL-7 R alpha / CD127 Protein, Fc Tag, Avi Tag (Avitag™), 25µg  

Recombinant Biotinylated Human IL-7 R alpha / CD127 Protein, Fc Tag, Avi Tag (Avitag™), 25µg

Biotinylated Human IL-7 RA / CD127 Protein (Glu 21 - Gly 236), expressed from human 293 cells (HEK293), Fc tag, Avi tag (Avitag™)

Synonym
recombinant, human, biotinylated, protein, IL7Ra,CD127

More details

IL7-H82F8-25

Availability: within 7 days

420,00 €

Background
Interleukin-7 receptor subunit alpha (IL-7 RA or IL7Ra) is also known as cluster of differentiation 127 (CD127). IL-7 RA is a type I cytokine receptor and is a subunit of the functional Interleukin-7 receptor and thymic stromal lymphopoietin (TSLP) receptors. IL-7 RA has been shown to play a critical role in the development of immune cells called lymphocytes - specifically in a process known as V(D)J recombination[citation needed]. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation.

Source
Recombinant Biotinylated Human IL-7 R alpha, Fc,Avitag (IL7-H82F8) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Gly 236 (Accession # P16871-1(I66T, V138I)).
Predicted N-terminus: Glu 21

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).
The protein has a calculated MW of 53.3 kDa. As a result of glycosylation, the protein migrates as 70-100 kDa under reducing (R) condition, and 130-200 kDa under non-reducing (NR) condition (SDS-PAGE).

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
Passed as determined by the HABA assay / binding ELISA.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
 
Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Plasma cytokines during pregnancy provide insight into the risk of diabetes in the gestational diabetes risk group"
Tagoma, Haller-Kikkatalo, Oras et al
J Diabetes Investig (2022)
(2) "Peripheral inflammatory cytokines and motor symptoms in persons with Parkinson's disease"
Diaz, Kohut, Russell et al
Brain Behav Immun Health (2022) 21, 100442
(3) "Honey Bee Products: Preclinical and Clinical Studies of Their Anti-inflammatory and Immunomodulatory Properties"
El-Seedi, Eid, Abd El-Wahed et al
Front Nutr (2021) 8, 761267
Showing 1-3 of 155 papers.