Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Biotinylated Human CD38 Protein, Avi Tag (Avitag™), His tag, 25 µg  

Recombinant Biotinylated Human CD38 Protein, Avi Tag (Avitag™), His tag, 25 µg

Recombinant biotinylated Human CD38, Val 43 - Ile 300, expressed from human 293 cells (HEK293), His tag, Avi tag (Avitag™)

Synonym: Recombinant human biotinylated Protein, CD38,T10,cADPr hydrolase 1

More details

CD8-H82E7-25

Availability: within 7 days

325,00 €

Background
CD antigen CD38 is also known as ADP-ribosyl cyclase 1, which belongs to the ADP-ribosyl cyclase family. CD38 is expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma. CD38 is a multifunctional ectoenzyme that catalyzes the synthesis and hydrolysis of cyclic ADP-ribose (cADPR) from NAD+ to ADP-ribose. These reaction products are essential for the regulation of intracellular Ca2+. The loss of CD38 function is associated with impaired immune responses, metabolic disturbances, and behavioral modifications. The CD38 protein is a marker of cell activation. It has been connected to HIV infection, leukemias, myelomas, solid tumors, type II diabetes mellitus and bone metabolism. CD38 has been used as a prognostic marker in leukemia.

Source
Recombinant Biotinylated Human CD38, Avitag,His Tag (CD8-H82E7) is expressed from human 293 cells (HEK293). It contains AA Val 43 - Ile 300 (Accession # NP_001766.2).
Predicted N-terminus: Val 43

Molecular Characterization
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 32.5 kDa. The protein migrates as 40-48 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
Passed as determined by the HABA assay / binding ELISA.

Endotoxin
Less than 1.0 EU per μg by the LAL method.
 
Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
 
Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Prognostic value of translocation 11;14 in patients with relapsed/refractory myeloma receiving anti-CD38 therapy"
Mohyuddin, Chakraborty, Calip et al
Blood Cancer J (2022) 12 (12), 168
(2) "Case report: Daratumumab treatment in pre-transplant alloimmunization and severe hemolytic anemia"
Pereda, Hosahalli Vasanna, Desai et al
Front Immunol (2022) 13, 1055473
(3) "Case report: CD38-directed CAR-T cell therapy: A novel immunotherapy targeting CD38- positive blasts overcomes TKI and chemotherapy resistance of myeloid chronic myeloid leukemia in blastic phase"
Cui, Liang, Dai et al
Front Immunol (2022) 13, 1012981
Showing 1-3 of 9205 papers.