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Recombinant human CSF1R /CD115 Protein, Fc Tag, 200µg  

Recombinant human CSF1R /CD115 Protein, Fc Tag, 200µg

Recombinant Human CSF1R /CD115 Protein, Ile 20 - Glu 512, produced in human 293 cells (HEK293), Fc Tag

Synonym
Recombinant Human Protein, CSF1R, C-FMS, CD115, CSFR, FIM2, FMS, M-CSFR

More details

CSR-H5258-200

Availability: within 7 days

336,00 €

Background
Colony stimulating factor 1 receptor (CSF1R), also known as macrophage colony-stimulating factor receptor (M-CSFR), CD115 Cluster of Differentiation 115 (CD115), C-FMS, CSFR, FIM2, FMS, and is a member of the type III subfamily of receptor tyrosine kinases (RTKs). CSF1R is a receptor for a cytokine called colony stimulating factor 1, The protein encoded by the CSFR1 gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most, if not all, of the biological effects of this cytokine. Ligand binding activates CSFR1 through a process of oligomerization and transphosphorylation. Mutations in CSF1R are associated with chronic myelomonocytic leukemia and type M4 acute myeloblastic leukemia. Increased levels of CSF1R1 are found in microglia in Alzheimer\'s disease and after brain injuries. The increased receptor expression causes microglia to become more active. Both CSF1R, and its ligand colony stimulating factor 1 play an important role in the development of the mammary gland and may be involved in the process of mammary gland carcinogenesis.

Source
Recombinant Human CSF1R /CD115 Protein, With C-Fc Tag (rhCSF1R-Fc) Ile 20 - Glu 512 (Accession # NP_005202.2) was produced in human 293 cells (HEK293).

Molecular Characterization
rhCSF1R-Fc, fused with Fc fragment of human IgG1 at the C-terminus, has a calculated MW of 80.7 kDa. The predicted N-terminus is Ile 20. DTT-reduced Protein migrates as 95-150 kDa due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCSF1R-Fc by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4C-8C); After reconstitution under sterile conditions for 1 month (4C-8C) or 3 months (-20C to -70C).

Bioactivity
Immobilized Human M-CSF, His Tag at 5 μg/mL (100 μL/well) can bind Human M-CSF R, Fc Tag (Cat. No. CSR-H5258) with a linear range of 0.1-4 ng/mL (QC tested)

References

(1) Ridge, et al., 1990, Proc Natl Acad Sci U S A., 87(4): 1377-80.
(2) Mitrasinovic, O. M., 2005, Journal of Neuroscience 25 (17): 4442–51.
(3) Tamimi, R. M., 2008, Cancer Research 68 (1): 18–21.
(4) Pollard, J. W., 1994, Proceedings of the National Academy of Sciences 91 (20): 9312–6.
(5) Sapi, Eva, 2004, Experimental Biology and Medicine 229 (1): 1–11.