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Recombinant Biotinylated Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein, His-tag, Avitag™&Tag Free, 25 µg  

Recombinant Biotinylated Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein, His-tag, Avitag™&Tag Free, 25 µg

Recombinant Biotinylated Human Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein, AA Phe 31 - Val 992 (ITGAV) & Gln 21 - Asp 728 (ITGB1), expressed from human 293 cells (HEK293), His-Tag, Avitag™&Tag Free

Synonym: Recombinant, human, protein, Integrin alpha V beta 1, ITGAV&ITGB1

More details

IT1-H82W6-25

Availability: within 7 days

455,00 €

Background
Integrin alpha-5/beta-1 is a receptor for ibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-V/beta-1 is also a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform 2 interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition.

Source
Recombinant Biotinylated Human ITGAV&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (IT1-H82W6) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Gln 21 - Asp 728 (ITGB1) (Accession # NP_002201.1 (ITGAV) & NP_002202.2 (ITGB1)).
Predicted N-terminus: Phe 31 (ITGAV) & Gln 21 (ITGB1)

Molecular Characterization
Biotinylated Human ITGAV&ITGB1 Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of ITGAV and ITGB1, has a calculated MW of 114.7 kDa (ITGAV) and 83.7 kDa (ITGB1). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 135-150 kDa (ITGAV) and 100-115 kDa (ITGB1) respectively due to glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
Passed as determined by the HABA assay / binding ELISA.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet

Clinical and Translational Updates

(1) "LQB-118 Suppresses Migration and Invasion of Prostate Cancer Cells by Modulating the Akt/GSK3β Pathway and MMP-9/Reck Gene Expression"
Martino, DE Bem, Santos et al
Anticancer Res (2023) 43 (1), 359-367
(2) "Evaluation of integrin αvβ3-targeted imaging for predicting disease progression in patients with high-risk differentiated thyroid cancer (using 99mTc-3PRGD2)"
Liang, Jia, Wang et al
Cancer Imaging (2022) 22 (1), 72
(3) "Lasso peptide microcin J25 variant containing RGD motif as a PET probe for integrin a v ß 3 in tumor imaging"
Mohri, Nhat, Zouda et al
Eur J Pharm Sci (2023) 180, 106339
Showing 1-3 of 1055 papers.

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