Recombinant Biotinylated Human ANGPTL7 / CDT6 Protein, Mouse IgG2a Fc-tg, Avitag™, 25 µg

Background
Angiopoietin-like 7 (ANGPTL7) is also known as Corneal-Derived Transcript 6 (CDT6), and is a secreted glycoprotein that is structurally related to the angiopoietins. It is expressed in the corneal stroma, trabecular meshwork, and sclera and is elevated in glaucoma aqueous humor. Recently, ANGPTL proteins, including ANGPTL-2, -3, -4, -5 and -7, are identified as growth factors in combination with SCF, Thrombopoietin, IGF-II and FGF acidic that enhance the expansion and engraftment of human and mouse hematopoietic stem cells.

Source
Recombinant Biotinylated Human ANGPTL7 protein, Mouse IgG2a Fc,Avitag (AN7-H82F3) is expressed from human 293 cells (HEK293). It contains AA Gln 27 - Pro 346 (Accession # O43827-1).
Predicted N-terminus: Gln 27

Molecular Characterization
This protein carries a mouse IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 65.8 kDa. The protein migrates as 60 kDa and 70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
Passed as determined by the HABA assay / binding ELISA.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "ANGPTL7 and Its Role in IOP and Glaucoma"
Brown, Nguyen, Mzyk et al
Invest Ophthalmol Vis Sci (2024) 65 (3), 22
(2) "Genes as drugs for glaucoma: latest advances"
Borrás, Stepankoff, Danias
Curr Opin Ophthalmol (2024) 35 (2), 131-137
(3) "Cord blood stem cell‑derived Angptl7 ameliorates the severity of bronchopulmonary dysplasia via anti‑inflammatory and proangiogenic effects"
Ren, Yang, Wang et al
Mol Med Rep (2024) 29 (1)
Showing 1-3 of 72 papers.