Recombinant human FcRn/FCGRT & B2M Heterodimer Protein, (SPR & BLI verified), 50µg

Background
FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

Source
Recombinant Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (FCM-H5286) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # P55899-1(FCGRT) & P61769-1(B2M)).
Predicted N-terminus: Ala 24 (FCGRT) & Ile 21 (B2M)

Molecular Characterization
Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 31.2 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The reducing (R) protein migrates as 33 kDa (FCGRT) and 13 kDa (B2M) respectively due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 12 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

BIAFFIN SERVICES:

Use BIAFFINS FcRn BINDING ASSAYS for detailed kinetic analysis of your antibodies using SPR-based assays.

Figure: Real-time kinetic analysis of antibody binding to FcRn using surface plasmon resonance.

Please contact our application specialists to obtain more information and an individual quote tailored to your specific needs.

Clinical and Translational Updates

(1) "Human FcRn Is a Two-in-One Attachment-Uncoating Receptor for Echovirus 18"
Chen, Qu, Liu et al
mBio (2022)
(2) "Detection of nucleotide variants in FCGRT (Fc fragment of IgG, receptor, transporter, alpha) gene and their influence on colostral IgG concentration in Indian water buffalo (Bubalus bubalis)"
Agrawal, Kumar, Chaudhary et al
Mol Biol Rep (2022) 49 (5), 3773-3781
(3) "Polymorphisms of neonatal Fc receptor in cynomolgus and rhesus macaques"
Uno, Utoh, Iwasaki
Drug Metab Pharmacokinet (2014) 29 (5), 427-30
Showing 1-3 of 4 papers.

Citations
(1) "Enhancing durability of CIS43 monoclonal antibody by Fc mutation or AAV delivery for malaria prevention"
Authors: Kielau Kisalu, Neville et al.
Journal: JCI Insight 2020
Application: BLI
(2) "Engineered antibody Fc variants for enhanced serum half life"
Authors: G Georgiou, et al
Journal: US20190048078A1 2019
Application: SPR
(3) "ontrol of therapeutic IgG antibodies galactosylation during cultivation process and its impact on IgG1/FcγR interaction and ADCC activity"
Authors: Simonov V, et al
Journal: Biologicals 2019
Application: BLI
Showing 1-3 of 6 papers.