Recombinant human Semaphorin 4D / SEMA4D / CD100 Protein, Fc Tag, 100µg

Background
Cluster of Differentiation 100 (CD100), also known as Semaphorin-4D (SEMA4D), is a single-pass type I membrane protein which belongs to the semaphorin family, and is a human 150-kDa homodimer expressed at the surface of most hemopoietic cells.[1] Semaphorin genes encode soluble and membrane-bound proteins, most of which have been shown to act as chemorepellents on growth cone guidance. CD100 is discrete, as it is a transmembrane leukocyte surface molecule that can also exist in a soluble form.[1] Semaphorin 4D (Sema 4D) is an axon guidance molecule which is secreted by oligodendrocytes and induces growth cone collapse in the central nervous system. By binding plexin B1 receptor it functions as an R-Ras GTPase-activating protein (GAP) and repels axon growth cones in both the mature central nervous system.[2] In the immune system, CD100 binds CD72 to activate B cells and dendritic cells, though much about this interaction is still under investigation.[3-4] It is involved in oligodendrogenesis during development and during recovery from ischemic injury.

Source
Recombinant human SEMA4D /CD100 Protein, Fc Tag (CD0-H5257) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Arg 734 (Accession # AAH54500).
Predicted N-terminus: Met 22

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 105.4 kDa. The protein migrates as 125-160 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20^°C to -70^°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Soluble Semaphorin 4D Serum Concentrations Are Elevated in Critically Ill Patients with Liver Cirrhosis and Correlate with Aminotransferases"
Abu Jhaisha, Hohlstein, Yagmur et al
Diagnostics (Basel) (2024) 14 (4)
(2) "Plexin-B1 and Plexin-B2 play non-redundant roles in GABAergic synapse formation"
Adel, Pranske, Kowalski et al
Mol Cell Neurosci (2024) 128, 103920
(3) "Schistosome egg-derived extracellular vesicles deliver Sja-miR-71a inhibits host macrophage and neutrophil extracellular traps via targeting Sema4D"
Liao, Zhu, Liu et al
Cell Commun Signal (2023) 21 (1), 366
Showing 1-3 of 381 papers.