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Recombinant Human Dkk-1 Protein, Fc Tag, 100 µg  

Recombinant Human Dkk-1 Protein, Fc Tag, 100 µg

Recombinant Human Dkk-1 Protein (Thr 32 - His 266), produced in human 293 cells (HEK293), Fc tag (MALS verified)

Synonym
Recombinant Human protein, DKK1,SK

More details

DK1-H5258-100

Availability: within 7 days

348,00 €

Background
Members of the dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. And DKK1 takes part in embryonic development through its inhibition of the WNT signaling pathway, binds to LRP6 with high affinity and prevents the Frizzled-Wnt-LRP6 complex formation in response to Wnts. DKK1 promotes LRP6 internalization and degradation when it forms a ternary complex with the cell surface receptor Kremen.DKK1 not olny functions as a head inducer during development, but also regulates joint remodeling and bone formation, which suggests roles for DKK1 in the pathogenesis of rheumatoid arthritis and multiple myeloma. More recently research reported, DKK1 impacts eye development from a defined developmental time point on, and is critical for lens separation from the surface ectoderm via β-catenin mediated Pdgfrα and E-cadherin expression.

Source
Recombinant Human Dkk-1 Protein, Fc Tag (DK1-H5258) is expressed from human 293 cells (HEK293). It contains AA Thr 32 - His 266 (Accession # NP_036374.1).
Predicted N-terminus: Thr 32

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 52.4 kDa. The protein migrates as 67-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Human Dkk-1, Fc Tag by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "High Dickkopf-1 (DKK-1) levels are associated with chronic inflammation in children with Sickle Cell Disease"
Giordano, Del Vecchio, Russo et al
Eur J Haematol (2021)
(2) "Dickkopf-1 Inhibition Reactivates Wnt/β-Catenin Signaling in Rhabdomyosarcoma, Induces Myogenic Markers In Vitro and Impairs Tumor Cell Survival In Vivo"
Giralt, Gallo-Oller, Navarro et al
Int J Mol Sci (2021) 22 (23)
(3) "The cost-effectiveness of oral semaglutide versus empagliflozin in Type 2 diabetes in Denmark"
Ehlers, Lamotte, Ramos et al
J Comp Eff Res (2022) 11 (1), 29-37
Showing 1-3 of 883 papers.