Products

Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Human CD4 Protein, Fc Tag (MALS verified) DMF Filed, 200µg  

Recombinant Human CD4 Protein, Fc Tag (MALS verified) DMF Filed, 200µg

Recombinant Human CD4 Protein, Lys 26 - Pro 396, produced in human 293 cells (HEK293), Fc Tag (MALS verified) DMF Filed

Synonym
Recombinant human protein, CD4, CD-4, CD4mut, CD-4mut, LEU3

More details

CD4-H5259-200

Availability: within 7 days

396,00 €

Background
T-cell surface glycoprotein CD4 is also known as T-cell surface antigen T4/Leu-3. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is accessory protein for MHC class-II antigen/T-cell receptor interaction. CD4 induces the aggregation of lipid rafts. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Therefore, medical professionals refer to the CD4 count to decide when to begin treatment for HIV-infected patients.

Source
Recombinant Human CD4, Fc Tag (MALS verified) (CD4-H5259) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Pro 396 (Accession # AAH25782).
Predicted N-terminus: Lys 26

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 67.5 kDa. The protein migrates as 70-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCD4-Fc by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris, Glycine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Evaluation of cytokine levels in response to mitogen among HIV-1-infected blood cells and their relationships to the number of T cells"
Imoto, Suzukawa, Takeda et al
Cytokine (2022) 153, 155840
(2) "Effect of major versus minor mastectomy on host immunity in canine mammary cancer"
Karayannopoulou, Anagnostou, Margariti et al
Vet Immunol Immunopathol (2022) 246, 110403
(3) "Expanded CD4+CXCR5-PD-1+ peripheral T helper like cells and clinical significance in IgG4-related disease"
Zhang, Wang, Chen et al
Clin Immunol (2022)
Showing 1-3 of 183159 papers.