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Recombinant Rhesus macaque CD4 Protein, His-Tag, 100µg  

Recombinant Rhesus macaque CD4 Protein, His-Tag, 100µg

Recombinant Rhesus macaque CD4 Protein, Lys 26 - Trp 390, produced in human 293 cells (HEK293), His Tag

Synonym
recombinant,protein, CD4,CD4mut,LEU3

More details

CD4-C52H7-100

Availability: within 7 days

300,00 €

Background
T-cell surface glycoprotein CD4 is also known as T-cell surface antigen T4/Leu-3. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is accessory protein for MHC class-II antigen/T-cell receptor interaction. CD4 induces the aggregation of lipid rafts. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Therefore, medical professionals refer to the CD4 count to decide when to begin treatment for HIV-infected patients.

Source
Rhesus macaque CD4 Protein (Rhesus macaque CD4, His Tag) Lys 26 - Trp 390 (Accession # G7N5T8) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization
Rhesus macaque CD4, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 42.3 kDa. The predicted N-terminus is Lys 26. The reducing (R) protein migrates as 52-60 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). 

References

(1) Brady RL, et al., 1993, Science 260 (5110): 979–83.
(2) Zeitlmann, L., et al., 2001, J. Biol. Chem. (United States) 276 (12): 9123–32.
(3) Rudd CE, et al., 2010, J. Immunol. 185 (5): 2645–9.
(4) Rudd CE, et al., 1988, Proc. Natl. Acad. Sci. U.S.A. 85 (14): 5190–4.
(5) Gorska MM, et al., 2004, J. Exp. Med. 199 (3): 369–79.