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Recombinant human CD3 Epsilon / CD3E Protein, His tag (MALS verified), 100µg  

Recombinant human CD3 Epsilon / CD3E Protein, His tag (MALS verified), 100µg

Recombinant Human CD3 Epsilon /CD3E Protein (Asp23-Asp126), produced in human 293 cells (HEK293), His tag (MALS verified)

Recombinant Human protein, FLJ18683, T3E, TCRE, CD3E, CD3-epsilon

More details


Availability: within 7 days

462,00 €

CD3e molecule, epsilon also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.[1-4]

Recombinant Human CD3 epsilon, His Tag (CDE-H5223) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (Accession # P07766-1).
Predicted N-terminus: Asp 23

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 16.9 kDa. As a result of glycosylation, the protein migrates as 20-21 kDa under reducing (R) condition, and 35-45 kDa under non-reducing (NR) condition (SDS-PAGE).

Less than 1.0 EU per μg of the rhCD3E by the LAL method.

>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Please refer to product data sheet.

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "[Bispecific antibodies targeting CD3 in oncology and hematology]"
Vanacker, Vinceneux, Nicolas-Virelizier et al
Bull Cancer (2021) 108 (10S), S181-S194
(2) "Cardiac inflammation and microvascular procoagulant changes are decreased in second wave compared to first wave deceased COVID-19 patients"
Wu, Baylan, van der Leeden et al
Int J Cardiol (2021)
(3) "Distinct mutational profile and immune microenvironment in microsatellite-unstable and POLE-mutated tumors"
Hwang, Kim, Choi
J Immunother Cancer (2021) 9 (10)
Showing 1-3 of 853 papers.


(1)"Multispecific immunomodulatory antigen-binding constructs"
Authors: TJ Schuetz, et al
Journal: US20190031785A1 2019
Application: BLI
(2) "Multispecific Immunomodulatory antigen-binding constructs"
Authors: TJ Schuetz, et al
Journal: US20180318417A1 2018
Application: BLI