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Recombinant human CD3 Epsilon / CD3E Protein, His tag (MALS verified), 100µg  

Recombinant human CD3 Epsilon / CD3E Protein, His tag (MALS verified), 100µg

Recombinant Human CD3 Epsilon /CD3E Protein (Asp23-Asp126), produced in human 293 cells (HEK293), His tag (MALS verified)

Synonym
Recombinant Human protein, FLJ18683, T3E, TCRE, CD3E, CD3-epsilon

More details

CDE-H5223-100

Availability: within 7 days

462,00 €

Background
CD3e molecule, epsilon also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.[1-4]

Source
Recombinant Human CD3 epsilon, His Tag (CDE-H5223) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (Accession # P07766-1).
Predicted N-terminus: Asp 23

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 16.9 kDa. As a result of glycosylation, the protein migrates as 20-21 kDa under reducing (R) condition, and 35-45 kDa under non-reducing (NR) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg of the rhCD3E by the LAL method.

Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Bioactivity
Please refer to product data sheet.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

References

(1) "Acute TBK1/IKK-ε Inhibition Enhances the Generation of Disease-Associated Microglia-Like Phenotype Upon Cortical Stab-Wound Injury"
Rehman, Tar, Olamide et al
Front Aging Neurosci (2021) 13, 684171
(2) "Comprehensive immunomolecular profiling of endometrial carcinoma: A tertiary retrospective study"
Victoor, Borght, Spans et al
Gynecol Oncol (2021)
(3) [Characteristics of primary cutaneous rare nasal extranodal NK/T cell lymphoma: a clinicopathological study of 15 cases]
Zhao, Zhao, Wang et al
Zhonghua Bing Li Xue Za Zhi (2021) 50 (6), 609-614
Showing 1-3 of 846 papers.
Please refer to product data sheet.