Recombinant human CD3 Epsilon / CD3E Protein, His tag (MALS verified), 100µg

Background
CD3e molecule, epsilon also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.

Source
Recombinant Human CD3 epsilon, His Tag (CDE-H5223) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (Accession # P07766-1).
Predicted N-terminus: Asp 23

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.9 kDa. Maybe caused by unknown PTMs, the protein migrates as 20-21 kDa under reducing (R) condition, and 35-45 kDa under non-reducing (NR) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Bioactivity
Please refer to product data sheet.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 24 months in lyophilized state;
-70^°C for 12 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "A PD-L1xCD3 bispecific nanobody as a novel T-cell engager in treating PD-L1 overexpression melanoma"
Li, Wang, Shan et al
Mol Immunol (2023) 163, 20-27
(2) "Genetic code expansion in E. coli enables production of a functional 'ready-to-click' T cell receptor-specific scFv"
Pasupuleti, Rosato, Kolanovic et al
N Biotechnol (2023) 76, 127-137
(3) "Structure-based engineering of a novel CD3ε-targeting antibody for reduced polyreactivity"
Liu, Ahonen, Brown et al
MAbs (2023) 15 (1), 2189974
Showing 1-3 of 864 papers.

Citations

(1)"Multispecific immunomodulatory antigen-binding constructs"
Authors: TJ Schuetz, et al
Journal: US20190031785A1 2019
Application: BLI
(2) "Multispecific Immunomodulatory antigen-binding constructs"
Authors: TJ Schuetz, et al
Journal: US20180318417A1 2018
Application: BLI