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Recombinant Cynomolgus CD3 epsilon Protein,  His tag (MALS verified), 100µg  

Recombinant Cynomolgus CD3 epsilon Protein, His tag (MALS verified), 100µg

Recombinant Cynomolgus CD3 epsilon Protein, AA Gln 22 - Asp 117, produced in human 293 cells (HEK293), His tag (MALS verified)

recombinant, cynomolgus, protein, FLJ18683, T3E, TCRE, CD3E, CD3-epsilon

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Availability: within 7 days

481,00 €

CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.

Recombinant Cynomolgus CD3 epsilon, His Tag (CDE-C5226) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (Accession # Q95LI5-1).
Predicted N-terminus: Gln 22

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 16.1 kDa. The protein migrates as 18-23 kDa under reducing (R) condition, and 33-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Less than 1.0 EU per μg by the LAL method.

>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

See Certificate of Analysis for reconstitution instructions and specific concentrations.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Please refer to product data sheet.

Clinical and Translational Updates

(1) "A PD-L1xCD3 bispecific nanobody as a novel T-cell engager in treating PD-L1 overexpression melanoma"
Li, Wang, Shan et al
Mol Immunol (2023) 163, 20-27
(2) "Genetic code expansion in E. coli enables production of a functional 'ready-to-click' T cell receptor-specific scFv"
Pasupuleti, Rosato, Kolanovic et al
N Biotechnol (2023) 76, 127-137
(3) "Structure-based engineering of a novel CD3ε-targeting antibody for reduced polyreactivity"
Liu, Ahonen, Brown et al
MAbs (2023) 15 (1), 2189974
Showing 1-3 of 864 papers.

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