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Recombinant Cynomolgus CD3 epsilon Protein, His Tag, 100µg  

Recombinant Cynomolgus CD3 epsilon Protein, His Tag, 100µg

Recombinant Cynomolgus CD3 epsilon Protein, Gln 22 - Asp 117, produced in human 293 cells (HEK293), His tag

Synonym
recombinant, cynomolgus, protein, T3E,TCRE,CD3E,CD3-epsilon

More details

CDE-C5226-100

Availability: within 7 days

420,00 €

Background
CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.

Source
Recombinant Cynomolgus CD3 epsilon, His Tag (CDE-C5226) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (Accession # Q95LI5-1). Predicted N-terminus: Gln 22

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 12.2 kDa. The protein migrates as 10 kDa and 14-21 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

Bioactivity
Immobilized Mouse Anti-Human CD3 (SP34-2) at 2 μg/mL (100 μL/well) can bind Cynomolgus CD3 epsilon, His Tag (Cat. No. CDE-C5226) with a linear range of 0.05-1.6 ng/mL (QC tested).
SP34-2 (Mouse IgG1) captured on CM5 chip via anti-mouse antibodies surface, can bind Cynomolgus CD3E, His Tag (Cat. No. CDE-C5226) with an affinity constant of 0.981 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

References

(1) Gold DP, et al., 1986, Nature 321 (6068): 431–4.
(2) Clevers HC, et al., 1988, Proc. Natl. Acad. Sci. U.S.A. 85 (21): 8156–60.
(3) de Saint Basile G, et al., 2004, J. Clin. Invest. 114 (10): 1512–7.
(4) Wong S, et al., 1991, Clin. Exp. Immunol. 83 (1): 69–73.