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Recombinant human CD33 / SIGLEC-3 Protein, His tag, 100µg  

Recombinant human CD33 / SIGLEC-3 Protein, His tag, 100µg

Recombinant Human CD33 / SIGLEC-3 Protein (Asp 18 - His 259 ), produced in human 293 cells (HEK293), His tag

Synonym
Recombinant human protein, CD33, SIGLEC3, gp67

More details

CD3-H5226-100

Availability: within 7 days

348,00 €

Background
Myeloid cell surface antigen CD33 also known as SIGLEC3, Siglecs (sialic acid binding Ig-like lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, one Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia. [1-5]

Source
Recombinant Human CD33 /SIGLEC-3 Protein (rhCD33) Asp 18 - His 259 (Accession # AAH28152.1) was produced in human 293 cells (HEK293).

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 27.6 kDa. The protein migrates as 43-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCD33 by the LAL method.

Purity
>95% purity as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Human Siglec-3, His Tag (Cat. No. CD3-H5226) ELISA bioactivity
Immobilized Anti-Human CD33 MAb, Mouse IgG1 at 2 μg/mL (100 μL/well) can bind Human Siglec-3, His Tag (Cat. No. CD3-H5226) with a linear range of 0.2-1 ng/mL (QC tested).

References

(1) Garnache-Ottou F., et al., 2005, Blood 105 (3): 1256–64.
(2) Hernandez-Caselles T, et al., 2006, J. Leukoc. Biol. 79 (1): 46–58.
(3) Walter RB, et al., 2007, Blood 109 (10): 4168–70.
(4) Ulyanova, T. et al., 1999, Eur. J. Immunol. 29:3440.
(5) Crocker, P.R. and A. Varki, 2001, Immunology 103:137.