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Recombinant human CD33 / SIGLEC-3 Protein, His tag, 100µg  

Recombinant human CD33 / SIGLEC-3 Protein, His tag, 100µg

Recombinant Human CD33 / SIGLEC-3 Protein (Asp 18 - His 259 ), produced in human 293 cells (HEK293), His tag (MALS verified)

Synonym
Recombinant human protein, CD33, SIGLEC3, gp67

More details

CD3-H5226-100

Availability: within 7 days

442,00 €

Background
Myeloid cell surface antigen CD33 also known as SIGLEC3, Siglecs (sialic acid binding Ig-like lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, one Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia. [1-5]

Source
Recombinant Human Siglec-3, His Tag (CD3-H5226) is expressed from human 293 cells (HEK293). It contains AA Asp 18 - His 259 (Accession # AAH28152.1).
Predicted N-terminus: Asp 18

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 27.6 kDa. The protein migrates as 43-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% purity as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "The impact of increasing levels of blood C-reactive protein on the inflammatory loci SPI1 and CD33 in Alzheimer's disease"
Huang, Tao, Ang et al
Transl Psychiatry (2022) 12 (1), 523
(2) "Peripheral level of CD33 and Alzheimer's disease: a bidirectional two-sample Mendelian randomization study"
Gu, Dou, Cao et al
Transl Psychiatry (2022) 12 (1), 427
(3) "Siglec-5 and Siglec-14 mediate the endocytosis of ADAMTS13"
Akiyama, Eura, Kokame
Thromb Res (2022) 219, 49-59
Showing 1-3 of 1075 papers.