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Recombinant Human Siglec-3 / CD33 Protein, Fc Tag, 100 µg  

Recombinant Human Siglec-3 / CD33 Protein, Fc Tag, 100 µg

Recombinant Human Siglec-3 / CD33 Protein, Asp 18 - His 259, produced in human 293 cells (HEK293), Fc Tag (MALS verified)

Synonym
recombinant, human, protein, CD33,SIGLEC3,gp67

More details

CD3-H5257-100

Availability: within 7 days

348,00 €

Background
Myeloid cell surface antigen CD33 is also known as SIGLEC3, Siglecs (sialic acid binding Ig­like lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-­terminal Ig-­like V­-type domain, one Ig­-like C2-­type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.

Source
Recombinant Human Siglec-3 / CD33 Protein, Fc Tag (CD3-H5257) is expressed from human 293 cells (HEK293). It contains AA Asp 18 - His 259 (Accession # AAH28152.1).
Predicted N-terminus: Asp 18

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 52.9 kDa. The protein migrates as 64-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Human Siglec-3, Fc Tag by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Bioactivity
Please refer to product data sheet.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Human brain sialoglycan ligand for CD33, a microglial inhibitory Siglec implicated in Alzheimer's disease"
Gonzalez-Gil, Porell, Fernandes et al
J Biol Chem (2022)
(2) "Primary CD33-targeting CAR-NK cells for the treatment of acute myeloid leukemia"
Albinger, Pfeifer, Nitsche et al
Blood Cancer J (2022) 12 (4), 61
(3) "CD33 rs2455069 SNP: Correlation with Alzheimer's Disease and Hypothesis of Functional Role"
Tortora, Rendina, Angiolillo et al
Int J Mol Sci (2022) 23 (7)
Showing 1-3 of 1055 papers.