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Recombinant Human Siglec-3 / CD33 Protein, Fc Tag, 100 µg  

Recombinant Human Siglec-3 / CD33 Protein, Fc Tag, 100 µg

Recombinant Human Siglec-3 / CD33 Protein, Asp 18 - His 259, produced in human 293 cells (HEK293), Fc Tag

Synonym
recombinant, human, protein, CD33,SIGLEC3,gp67

More details

CD3-H5257-100

Availability: within 7 days

348,00 €

Background
Myeloid cell surface antigen CD33 is also known as SIGLEC3, Siglecs (sialic acid binding Ig­like lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-­terminal Ig-­like V­-type domain, one Ig­-like C2-­type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.

Source
Recombinant Human Siglec-3 / CD33 Protein, Fc Tag (Human Siglec-3, Fc Tag) Asp 18 - His 259 (Accession # AAH28152.1) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization
Human Siglec-3, Fc Tag is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 52.9 kDa. The predicted N-terminus is Asp 18. DTT-reduced Protein migrates as 64-85 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Human Siglec-3, Fc Tag by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Bioactivity
Immobilized Human Siglec-3, Fc Tag (Cat. No. CD3-H5257) at 1 μg/mL (100 μL/well) can bind Anti-Human Anti-CD33 Mab with a linear range of 0.156-2.5 μg/mL (QC tested).

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

References

(1) Garnache-Ottou F., et al., 2005, Blood 105 (3): 1256–64.
(2) Hernández-Caselles T, et al., 2006, J. Leukoc. Biol. 79 (1): 46–58.
(3) Walter RB, et al., 2007, Blood 109 (10): 4168–70.
(4) Ulyanova, T. et al., 1999, Eur. J. Immunol. 29:3440.
(5) Crocker, P.R. and A. Varki, 2001, Immunology 103:137.