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Recombinant human PECAM1 / CD31 Protein, His tag, 100µg  

Recombinant human PECAM1 / CD31 Protein, His tag, 100µg

Recombinant Human CD31 /PECAM1 Protein (Gln 28 - Lys 601), produced in human 293 cells (HEK293), His tag

Synonym
Recombinant Human Protein, PECAM1, CD31, FLJ34100, FLJ58394

More details

CD1-H5224-100

Availability: within 7 days

300,00 €

Background
Cluster of Differentiation 31 (CD31), also known as Platelet endothelial cell adhesion molecule (PECAM-1), is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, macrophages and Kupffer cells, granulocytes, T / NK cells, lymphocytes, megakaryocytes, osteoclasts, neutrophils, certain tumors, and is the only known member of the CAM family on platelets. CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. CD31 plays a key role in removing aged neutrophils from the body. CD31 mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development. In addition, it has been shown that CD31 expression is up-regulated by LPS stimulation, and might function as a feedback negative regulator of LPS inflammatory response in macrophages.

Source
Recombinant Human CD31 /PECAM1 Protein (rhCD31) Gln 28 - Lys 601 (Accession # AAH22512.1) was produced in human 293 cells (HEK293).

Molecular Characterization
Human CD31, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 65.6 kDa. The predicted N-terminus is Gln 28. DTT-reduced Protein migrates as 100-110 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCD31 by the LAL method.

Purity
>98% purity as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 . Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Newman PJ, Newman DK., 2004, Arterioscler. Thromb. Vasc. Biol. 23 (6): 953–64.
(2) Kalinowska A, Losy J., 2007, Eur. J. Neurol. 13 (12): 1284–90.
(3) Almendro N, et al., 2007, J. Immunol. 157 (12): 5411–21.