Recombinant human PECAM1 / CD31 Protein, His tag, 100µg

Background
Cluster of Differentiation 31 (CD31), also known as Platelet endothelial cell adhesion molecule (PECAM-1), is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, macrophages and Kupffer cells, granulocytes, T / NK cells, lymphocytes, megakaryocytes, osteoclasts, neutrophils, certain tumors, and is the only known member of the CAM family on platelets. CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. CD31 plays a key role in removing aged neutrophils from the body. CD31 mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development. In addition, it has been shown that CD31 expression is up-regulated by LPS stimulation, and might function as a feedback negative regulator of LPS inflammatory response in macrophages.

Source
Recombinant Human CD31 /PECAM1 Protein (rhCD31), His Tag (CD1-H5224) is expressed from human 293 cells (HEK293). It contains AA Gln 28 - Lys 601 (Accession # P16284-1).
Predicted N-terminus: Gln 28

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 66.4 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 . Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Effectiveness of topical administration of platelet-rich plasma on the healing of methicillin-resistant Staphylococcus aureus-infected full-thickness wound model"
Leisi, Farahpour
J Plast Reconstr Aesthet Surg (2022) 77, 416-429
(2) "β-catenin/TCF4 inhibitors ICG-001 and LF3 alleviate BDL-induced liver fibrosis by suppressing LECT2 signaling"
Gao, Fan, Zhao et al
Chem Biol Interact (2023)
(3) "Targeting soluble epoxide hydrolase promotes osteogenic-angiogenic coupling via activating SLIT3/HIF-1α signalling pathway"
Gao, Chen, Li et al
Cell Prolif (2023)
Showing 1-3 of 17393 papers.