Recombinant Rhesus Macaque TNFRSF4 /OX40/CD134 Protein, His tag, 100µg

Background
Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) is also known as ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, CD antigen CD134, OX40. OX40 / TNFRSF4 contains four TNFR-Cys repeats. TNFRSF4 is receptor for TNFSF4 / OX40L / GP34 and can interacts with TRAF2, TRAF3 and TRAF5.

Source
Recombinant Rhesus macaque OX40, His Tag (OX0-C5220) is expressed from human 293 cells (HEK293). It contains AA Lys 28 - Ala 214 (Accession # XP_001090870.1). Predicted N-terminus: Lys 28

Molecular Characterization
Rhesus Macaque TNFRSF4 / OX40 / CD134 is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 22.1 kDa. The predicted N-terminus is Lys 28. DTT-reduced Protein migrates as 40-45 kDa in SDS-PAGE

Endotoxin
Less than 1.0 EU per μg of the Rhesus Macaque TNFRSF4 / OX40 / CD134 by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 3 months (-20°C to -70°C).

Bioactivity
Please refer to product data sheet.

References

(1) Arch R.H., et al., 1998, Mol. Cell. Biol. 18:558-565.(2) Kawamata S., et al., 1998, J. Biol. Chem. 273:5808-5814.