Recombinant Mouse TNFRSF4 /OX40/CD134 Protein, His tag, 100µg

Background
Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) is also known as ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, CD antigen CD134, OX40. OX40 / TNFRSF4 contains four TNFR-Cys repeats. TNFRSF4 is receptor for TNFSF4 / OX40L / GP34 and can interacts with TRAF2, TRAF3 and TRAF5

Source
Recombinant Mouse TNFRSF4 /OX40/CD134 Protein (Mouse TNFRSF4 / OX40 / CD134) Val 20 - Pro 211 (Accession # NP_035789) was produced in human 293 cells (HEK293)

Molecular Characterization
Mouse TNFRSF4 / OX40 / CD134 is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 23.2 kDa. The predicted N-terminus is Val 20. DTT-reduced Protein migrates as 45 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Mouse TNFRSF4 / OX40 / CD134 by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 3 months (-20°C to -70°C).

Bioactivity
Immobilized Mouse OX40, His Tag (Cat# OX0-M5228) at 2 μg/mL (100 μl/well) can bind Mouse OX40 Ligand, Fc Tag with a linear range of 2-30 ng/mL.

References