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Recombinant Human DNAM-1 / CD226 Protein, Fc Tag, 100µg  

Recombinant Human DNAM-1 / CD226 Protein, Fc Tag, 100µg

Recombinant Human DNAM-1 / CD226 Protein, Glu 19 - Asn 247, produced in human 293 cells (HEK293), Fc Tag

Synonym
recombinant, human, protein, DNAM1,CD226

More details

DN1-H5257-100

Availability: within 7 days

300,00 €

Background
DNAX accessory molecule 1 (DNAM-1), a single-pass type I membrane protein, is also known as CD226 antigen and platelet and T cell activation antigen 1 (PTA1), which contains 2 Ig-like C2-type (immunoglobulin-like) domains. DNAM-1 is a ~65 kDa glycoprotein expressed on the surface of natural killer cells, platelets, monocytes and a subset of T cells. DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.

Source
Recombinant Human DNAM-1 /CD226 Protein,C-Fc Tag (rh DNAM-1 / CD226 Fc Chimera) Glu 19 - Asn 247 (Accession # NP_006557) was produced in human 293 cells (HEK293).

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 52.6 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh DNAM-1 / CD226 Fc Chimera by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Human DNAM-1, Fc Tag (Cat# DN1-H5257) at 10 μg/mL (100 µl/well),can bind Human Nectin-2, His Tag with a linear range of 0.08-1.25 μg/mL.
Measured by its binding ability in a functional ELISA. Immobilized Human CD155, His Tag (Catalog # CD5-H5223) at 4 μg/mL (100 μL/well) can bind Human DNAM-1, Fc Tag (Catalog # DN1-H5257) with a linear range of 0.05-0.5 μg/mL.

References

(1) Shibuya A., et al.,1995, Immunity 4:573-581.
(2) Tahara-Hanaoka S., et al .,2003, Int. Immunol. 16:533-538.
(3) Pende D., et al.,2004, Mol. Immunol. 42:463-469.