Products

Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Human DNAM-1 /CD226 Protein, His Tag, 100µg  

Recombinant Human DNAM-1 /CD226 Protein, His Tag, 100µg

Recombinant Human DNAM-1 /CD226 Protein, Glu 19 - Asn 247, produced in human 293 cells (HEK293), His Tag

Synonym
recombinant, human protein DNAM1,CD226

More details

DN1-H52H6-100

Availability: within 7 days

300,00 €

Background
DNAX accessory molecule 1 (DNAM-1), a single-pass type I membrane protein, is also known as CD226 antigen and platelet and T cell activation antigen 1 (PTA1), which contains 2 Ig-like C2-type (immunoglobulin-like) domains. DNAM-1 is a ~65 kDa glycoprotein expressed on the surface of natural killer cells, platelets, monocytes and a subset of T cells. DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.

Source
Recombinant Human DNAM-1 /CD226 Protein (rh DNAM-1 / CD226) Glu 19 - Asn 247 (Accession # NP_006557) was produced in human 293 cells (HEK293).

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 27.9 kDa. The protein migrates as 36-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh DNAM-1 / CD226 by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization..

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Bioactivity
Immobilized Human DNAM-1, His Tag (Cat. No. DN1-H52H6) at 2μg/mL (100 μL/well) can bind Human CD155, Fc Tag (Cat. No. CD5-H5251) with a linear range of 3.3-51.2 ng/mL (QC tested).

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Shibuya A., et al.,1995, Immunity 4:573-581.
(2) Tahara-Hanaoka S., et al .,2003, Int. Immunol. 16:533-538.
(3) Pende D., et al.,2004, Mol. Immunol. 42:463-469.