Recombinant Human DNAM-1 /CD226 Protein, His Tag, 100µg

Background
DNAX accessory molecule 1 (DNAM-1), a single-pass type I membrane protein, is also known as CD226 antigen and platelet and T cell activation antigen 1 (PTA1), which contains 2 Ig-like C2-type (immunoglobulin-like) domains. DNAM-1 is a ~65 kDa glycoprotein expressed on the surface of natural killer cells, platelets, monocytes and a subset of T cells. DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.

Source
Recombinant Human DNAM-1, His Tag (DN1-H52H6) is expressed from human 293 cells (HEK293). It contains AA Glu 19 - Asn 247 (Accession # NP_006557).
Predicted N-terminus: Glu 19

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 27.9 kDa. The protein migrates as 36-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Bioactivity
Please refer to product data sheet.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 12 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "The central role of natural killer cells in mediating acute myocarditis after mRNA COVID-19 vaccination"
Tsang, Kwan, Chua et al
Med (2024)
(2) "Helicobacter pylori enhances HLA-C expression in the human gastric adenocarcinoma cells AGS and can protect them from the cytotoxicity of natural killer cells"
Apoorva, Jacob, Rao et al
Helicobacter (2024) 29 (2), e13069
(3) "A TEMPORAL DEVELOPMENTAL MAP SEPARATES HUMAN NK CELLS FROM NON-CYTOTOXIC ILCS THROUGH CLONAL AND SINGLE-CELL ANALYSIS"
Vo, Yuan, Kanaya et al
Blood Adv (2024)
Showing 1-3 of 444 papers.