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Recombinant Human IL-21 R Protein, His tag, 200 µg  

Recombinant Human IL-21 R Protein, His tag, 200 µg

Recombinant Human IL-21 R Protein (Cys 20 - Pro 236), produced in human 293 cells (HEK293), His tag

Synonym
Recombinant human protein, IL21R,CD360,NILR

More details

ILR-H5226-200

Availability: within 7 days

312,00 €

Background
Interleukin-21 receptor (IL21R) is also known as CD antigen CD360, Novel interleukin receptor (NILR), is a receptor for interleukin-21 and belongs to the type I cytokine receptor family or type 4 subfamily. IL21R contains two fibronectin type-III domains. IL21R is selectively expressed in lymphoid tissues and most highly expressed in thymus and spleen. IL21R transduces the growth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells.

Source
Recombinant Human IL-21 R Protein (Human IL-21 R, His Tag) Cys 20 - Pro 236 (Accession # AAH04348) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 25.8 kDa. The protein migrates as 36-46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>92% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

Bioactivity
Immobilized Biotinylated Human IL-21, Fc,Avitag (Cat. No. IL1-H82F7) at 2 μg/mL (100 μL/well) can bind Human IL-21 R, His Tag (Cat. No. ILR-H5226) with a linear range of 2-8 ng/mL (QC tested).

References

(1) Hamming O.J., et al., 2012, J. Biol. Chem. 287:9454-9460.
(2) Kotlarz D., et al., 2013, J. Exp. Med. 210:433-443.