Recombinant Human CD72 Protein, Fc Tag, 100 µg

Background
B-cell differentiation antigen CD72 is also known as Lyb-2. CD72 contains one C-type lectin domain. CD72 is a protein active in the immune system of animals. CD72 consists of two identical halves, each of about 39-43 kD, and is a C-type lectin. Its primarily locus of expression is B-cells, where it appears to mediate aspects of B-cell - T-cell interaction. CD72 plays a role in B-cell proliferation and differentiation. CD72 is a ligand for CD5.

Source
Recombinant Human CD72 Protein, Fc Tag (CD2-H5251) is expressed from human 293 cells (HEK293). It contains AA Arg 117 - Asp 359 (Accession # AAH30227).
Predicted N-terminus: Arg 117

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 54.2 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Phosphorylation stabilized TET1 acts as an oncoprotein and therapeutic target in B cell acute lymphoblastic leukemia"
Chen, Zhou, Xue et al
Sci Transl Med (2023) 15 (689), eabq8513
(2) "Regularized survival learning and cross-database analysis enabled identification of colorectal cancer prognosis-related immune genes"
Ai, Wang, Zhang et al
Front Genet (2023) 14, 1148470
(3) "Truncated-semaphorin3A is a potential regulatory molecule to restore immune homeostasis in immune-mediated diseases"
Eiza, Kessler, Sabag et al
Front Pharmacol (2022) 13, 1085892
Showing 1-3 of 305 papers.