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Recombinant Human CD72 Protein, Fc Tag, 100 µg  

Recombinant Human CD72 Protein, Fc Tag, 100 µg

Recombinant Human CD72 Protein (Arg 117 - Asp 359), produced in human 293 cells (HEK293), Fc tag

Synonym
Recombinant Human protein, CD72, Lyb-2, Lyb2

More details

CD2-H5251-100

Availability: within 7 days

348,00 €

Background
B-cell differentiation antigen CD72 is also known as Lyb-2. CD72 contains one C-type lectin domain. CD72 is a protein active in the immune system of animals. CD72 consists of two identical halves, each of about 39-43 kD, and is a C-type lectin. Its primarily locus of expression is B-cells, where it appears to mediate aspects of B-cell - T-cell interaction. CD72 plays a role in B-cell proliferation and differentiation. CD72 is a ligand for CD5.

Source
Recombinant Human CD72 Protein, With C-Fc Tag (rh CD72 Fc Chimera) Arg 117 - Asp 359 (Accession # AAH30227) was produced in human 293 cells (HEK293) at ACRObiosystems.

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 54.0 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh CD72 Fc Chimera by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

References

(1) von Hoegen I., et al., 1990, J. Immunol. 144:4870-4877.