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Recombinant Human Glypican 3 / GPC3 Protein, low endotoxin, His tag, 100 µg  

Recombinant Human Glypican 3 / GPC3 Protein, low endotoxin, His tag, 100 µg

Recombinant Human Glypican 3 / GPC3 (S359F) Protein, AA Gln 25 - His 559, expressed from human 293 cells (HEK293), low endotoxin

Synonym
Recombinant Human protein, GPC3,OCI5,Glypican-3,GTR2-2,MXR7,DGSX,SDYS ,SGB,SGBS,SGBS1

More details

GP3-H5223-100

Availability: within 7 days

384,00 €

Background
Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

Source
Recombinant Human Glypican 3 (S359F), low endotoxin (GP3-H5223) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # P51654 (S359F)).
Predicted N-terminus: Gln 25

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 61.7 kDa. The protein migrates as 66-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation and associated via disulfide bonds.

Endotoxin
Less than 0.1 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

Bioactivity-ELISA
Immobilized Human FGF basic, Tag Free (Cat. No. BFF-H4117) at 0.5 μg/mL (100 μL/well) can bind Human Glypican 3 (S359F), His Tag, low endotoxin (Cat. No. GP3-H5223) with a linear range of 3-128 ng/mL (QC tested).

Immobilized Human Glypican 3 (S359F), His Tag, low endotoxin (Cat. No. GP3-H5223) at 0.5 μg/mL (100 μL/well) can bind Anti-Human GPC3 Mab with a linear range of 0.4-3 ng/mL (Routinely tested).

Bioactivity-SPR
Anti-Human GPC3 MAb (chimeric mouse-human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human Glypican 3 (S359F), His Tag, low endotoxin (Cat. No. GP3-H5223) with an affinity constant of 1.96 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Loaded Anti-Human GPC3 MAb (chimeric mouse-human IgG1) on AHC Biosensor, can bind Human Glypican 3 (S359F), His Tag, low endotoxin (Cat. No. GP3-H5223) with an affinity constant of 3.04 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

References

(1) Davoodi J., et al., 2007, Proteomics 7:2300-2310.
(2) Veugelers M., et al., 2000, Hum. Mol. Genet. 9:1321-1328.