Recombinant Biotinylated Human GPA33, ultra sensitivity (primary amine labeling), His-Tag, 25 µg

Background
Glycoprotein A33 (GPA33) is also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling.

Source
MABSol ® Recombinant Biotinylated Human GPA33, His Tag, primary amine labeling (GP3-H8224) is expressed from human HEK293 cells. It contains AA Ile 22 - Val 235 (Accession # AAH74830). It is the biotinylated form of Human GPA33, His Tag (GP3-H5224).
Predicted N-terminus: Ile 22

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 24.4 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Biotin:Protein Ratio
Passed as determined by the HABA assay / binding ELISA.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Single-Cell Transcriptomics Reveals Discrete Steps in Regulatory T Cell Development in the Human Thymus"
Morgana, Opstelten, Slot et al
J Immunol (2022) 208 (2), 384-395
(2) "Intraperitoneal Pretargeted Radioimmunotherapy for Colorectal Peritoneal Carcinomatosis"
Chandler, Bell, Chung et al
Mol Cancer Ther (2022) 21 (1), 125-137
(3) "An integrated magneto-electrochemical device for the rapid profiling of tumour extracellular vesicles from blood plasma"
Park, Park, Huang et al
Nat Biomed Eng (2021) 5 (7), 678-689
Showing 1-3 of 104 papers.