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Recombinant Mouse M-CSF R / CSF1R / CD115 Protein, Fc Tag, 100 µg  

Recombinant Mouse M-CSF R / CSF1R / CD115 Protein, Fc Tag, 100 µg

Recombinant Mouse M-CSF R (Ala 20 - Ser 511), expressed from human 293 cells (HEK293), Fc tag

Synonym
Recombinant mouse protein, CSF1R,C-FMS,CD115,CSFR,FIM2,FMS,M-CSFR

More details

CSR-M5256-100

Availability: within 7 days

312,00 €

Background
Colony stimulating factor 1 receptor (CSF1R) is also known as macrophage colony-stimulating factor receptor (M-CSFR), CD115 Cluster of Differentiation 115 (CD115), C-FMS, CSFR, FIM2, FMS, and is a member of the typeⅢ subfamily of receptor tyrosine kinases (RTKs). CSF1R is a receptor for a cytokine called colony stimulating factor 1, The protein encoded by the CSFR1 gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most, if not all, of the biological effects of this cytokine. Ligand binding activates CSFR1 through a process of oligomerization and transphosphorylation . Mutations in CSF1R are associated with chronic myelomonocytic leukemia and type M4 acute myeloblastic leukemia. Increased levels of CSF1R1 are found in microglia in Alzheimer's disease and after brain injuries. The increased receptor expression causes microglia to become more active. Both CSF1R, and its ligand colony stimulating factor 1 play an important role in the development of the mammary gland and may be involved in the process of mammary gland carcinogenesis.

Source
Recombinant Mouse M-CSF R, Fc Tag (CSR-M5256) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Ser 511 (Accession # NP_001032948).
Predicted N-terminus: Ala 20

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 81.8 kDa. As a result of glycosylation, The protein migrates as 100-130 kDa under reducing (R) condition and 260 kDa under non-reducing (NR) condition on SDS-PAGE gel.

Endotoxin
Less than 0.1 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity (ELISA bioactivity)
Immobilized Mouse M-CSF Protein at 2μg/mL (100 μL/well) can bind Mouse M-CSF R, Fc Tag (Catalog # CSR-M5256) with a linear range of 0.3-12.8 ng/mL.

References

(1) Ridge, et al., 1990, Proc Natl Acad Sci U S A., 87(4): 1377-80.
(2) Mitrasinovic, O. M., 2005, Journal of Neuroscience 25 (17): 4442–51.
(3) Tamimi, R. M., 2008, Cancer Research 68 (1): 18–21.
(4) Pollard, J. W., 1994, Proceedings of the National Academy of Sciences 91 (20): 9312–6.
(5) Sapi, Eva, 2004, Experimental Biology and Medicine 229 (1): 1–11.