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Recombinant Human CD3 epsilon & CD3 gamma Heterodimer Protein, 50 µg  

Recombinant Human CD3 epsilon & CD3 gamma Heterodimer Protein, 50 µg

Recombinant Human CD3 epsilon & CD3 gamma Heterodimer Protein, Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G), produced in human 293 cells (HEK293), Fc tag, His tag (CD3E), Fc tag, Flag tag (CD3G) (MALS verified)

Synonym
Recombinant human protein, CD3 epsilon & CD3 gamma, CD3E & CD3G

More details

CDG-H52W9-50

Availability: within 7 days

455,00 €

Background
T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Source
Recombinant Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag(MALS verified) (CDG-H52W9) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # P07766-1(CD3E) & P09693-1(CD3G)).
Predicted N-terminus: Asp 23 (CD3E) & Gln 23 (CD3G)

Molecular Characterization
Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 39.2 kDa (CD3E) and 38.6 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag, and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus, followed by a Flag tag. The predicted N-terminus is Asp 23 (CD3E) & Gln 23 (CD3G). The reducing (R) heterodimer protein migrates as 40-50 kDa due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Lower BCL11B expression is associated with adverse clinical outcome for patients with myelodysplastic syndrome
Huang, Chen, Zhong et al
Biomark Res (2021) 9 (1), 46
(2) "Establishment and validation of immune microenvironmental gene signatures for predicting prognosis in patients with head and neck squamous cell carcinoma"
Wang, Tian, Zhu et al
Int Immunopharmacol (2021) 97, 107817
(3) "Identification of Hub Genes and Pathways of Triple Negative Breast Cancer by Expression Profiles Analysis"
Li, Huang, Zhu et al
Cancer Manag Res (2021) 13, 2095-2104
Showing 1-3 of 19 papers.