Recombinant human CD28H / IGPR-1 Protein, Fc Tag, 100 µg

Background
CD28 homolog (CD28H), also called transmembrane and immunoglobulin domain containing 2 (TMIGD2) and IGPR-1,is encoded by the TMIGD2 gene. CD28H is constitutively expressed on naive T and NK cells. After interaction of CD28 homolog with B7 homologue,the peripheral effector and memory T cells can be activated and proliferated by Akt-dependent signalling cascade. Plays a role in cell-cell interaction, cell migration, and angiogenesis. Through interaction with HHLA2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.

Source
Recombinant Human CD28H / IGPR-1 protein, Fc Tag (CDH-H5251) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Gly 150 (Accession # AAH15655.1).
Predicted N-terminus: Leu 23

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 40.6 kDa. The protein migrates as 50-60 kDa under reducing (R) condition, and 90-116 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.
 
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "NK Cells Equipped With a Chimeric Antigen Receptor That Overcomes Inhibition by HLA Class I for Adoptive Transfer of CAR-NK Cells"
Zhuang, Long
Front Immunol (2022) 13, 840844
(2) "Immunomodulation of T- and NK-cell Responses by a Bispecific Antibody Targeting CD28 Homolog and PD-L1"
Ramaswamy, Kim, Jones et al
Cancer Immunol Res (2022) 10 (2), 200-214
(3) "B7-H7 Is Inducible on T Cells to Regulate Their Immune Response and Serves as a Marker for Exhaustion"
Luu, Schwarz, Lundqvist
Front Immunol (2021) 12, 682627
Showing 1-3 of 15 papers.