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Recombinant Human CD73 Protein, Mouse IgG2a Fc Tag, 100µg  

Recombinant Human CD73 Protein, Mouse IgG2a Fc Tag, 100µg

Recombinant human CD73 Protein, Mouse IgG2a Fc Tag, AA Trp 27 - Ser 549, expressed in human 293 cells (HEK293), Fc tag

Synonym
Recombinant human protein,CD73, NT5E, 5'-Nucleotidase, 5'-NT, NT5, NTE

More details

CD3-H5252-100

Availability: within 7 days

420,00 €

Background
5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.

Source
Recombinant Human CD73, Mouse IgG2a Fc Tag (CD3-H5252) is expressed from human 293 cells (HEK293). It contains AA Trp 27 - Ser 549 (Accession # P21589-1).Predicted N-terminus: Trp 27

Molecular Characterization
This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 84.9 kDa. The protein migrates as 95-100 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCD33 by the LAL method.

Purity
>95% purity as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 51 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally Trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution..

Bioactivity
Please refer to product data sheet.