Recombinant Human IL-2 R beta&IL-2 R gamma Heterodimer Protein, His Tag&Twin-Strep Tag (MALS verified), 100µg

Background
Both Interleukin-2 receptor subunit beta and Interleukin-2 receptor subunit gamma are receptor for interleukin-2. Common subunit for the receptors for a variety of interleukins. Interacts with SHB upon interleukin stimulation. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.

Source
Recombinant Human IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag (ILG-H5283) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Asp 239 (IL-2RB) & Leu 23 - Asn 254 (IL-2RG) (Accession # P14784-1(IL-2RB) & P31785-1(IL-2RG)).
Predicted N-terminus: Ala 27 (IL-2RB) & Leu 23 (IL-2RG)

Molecular Characterization
Human IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag is produced by co-expression of IL-2RB and IL-2RG, has a calculated MW of 29.9 kDa (IL-2RB) and 34.1 kDa (IL-2RG). Subunit IL-2RB is fused with a polyhistidine tag at the C-terminus and subunit IL-2RG is fused with Twin-Strep tag at the C-terminus. The protein migrates as 40-44 kDa and 60-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "[Microwave ablation enhances the systemic immune response in patients with lung cancer"
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Oncol Lett (2024) 27 (3), 106
(2) "[Expression and Prognostic Value of Cytokines in Patients with Newly Diagnosed Diffuse Large B-Cell Lymphoma]"
Man, Ge, Li et al
Zhongguo Shi Yan Xue Ye Xue Za Zhi (2023) 31 (4), 1050-1055
(3) "Cytokine Responses during Mycobacterium tuberculosis H37Rv and Ascaris lumbricoides Costimulation Using Human THP-1 and Jurkat Cells, and a Pilot Human Tuberculosis and Helminth Coinfection Study"
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Microorganisms (2023) 11 (7)
Showing 1-3 of 296 papers.