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Recombinant human B7-H2 / ICOSLG Protein, 200µg  

Recombinant human B7-H2 / ICOSLG Protein, 200µg

Recombinant human B7-H2 / ICOSLG Protein (Human B7-H2, His Tag) Asp 19 - Ser 258 was produced in human 293 cells (HEK293).

Synonym: recombinant human ICOSLG,B7-H2,B7H2,B7RP-1,ICOS ligand

More details

B72-H5221-200

Availability: within 7 days

324,00 €

Background
ICOS ligand (ICOSLG) is also known as B7 homolog 2 (B7-H2), B7-related protein 1 (B7RP-1) and CD antigen CD275, which belongs to the immunoglobulin superfamily and BTN/MOG family. ICOSLG contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. Isoform 1 is widely expressed, while isoform 2 is detected only in lymph nodes, leukocytes and spleen. B7-H2 is ligand for the T-cell-specific cell surface receptor ICOS. B7-H2 acts as a costimulatory signal for T-cell proliferation and cytokine secretion and induces also B-cell proliferation and differentiation into plasma cells. B7-H2 could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.

Source
Recombinant human B7-H2 / ICOSLG Protein (Human B7-H2, His Tag) Asp 19 - Ser 258 was produced in human 293 cells (HEK293).

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 27.5 kDa. The protein migrates as 45-67 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Human B7-H2, His Tag by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Bioactivity
Immobilized Human ICOS, Fc Tag (Cat. No. ICS-H5258) at 1 μg/mL (100 μL/well) can bind Human B7-H2, His Tag (Cat. No. B72-H5221) with a linear range of 5-78 ng/mL (QC tested).

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Aicher A., et al., 2000, J. Immunol. 164:4689-4696.
(2) Yoshinaga SK., et al., Int Immunol 12 (10): 1439–47.