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Recombinant human CD48 / SLAMF2 Protein, 100µg  

Recombinant human CD48 / SLAMF2 Protein, 100µg

Recombinant human CD48 / SLAMF2 Protein (Human CD48, His Tag) Gln 27 - Ser 220 was produced in human 293 cells (HEK293) His Tag.

Synonym: recombinant human protein CD48,BCM1,SLAMF2,BLAST,BLAST1,MEM-102,TCT.1,BCM-1,SLAMF-2,BLAST-1

More details

BC1-H5226-100

Availability: within 7 days

300,00 €

Background
CD48 antigen is also known as Signaling lymphocytic activation molecule 2, SLAM family member 2 (SLAMF2), Leukocyte antigen MEM-102, BCM1 surface antigen, B-lymphocyte activation marker BLAST-1. CD48 is a 65 kDa GPI-linked protein which belongs to the CD2 subfamily of immunoglobulin superfamily molecules. CD48 antigen contains two Ig-like C2-type (immunoglobulin-like) domains and two Ig-like V-type (immunoglobulin-like) domain. CD48 interacts with CD244 in an heterophilic manner. CD48 is ligand for CD2. CD48 might facilitate interaction between activated lymphocytes. CD48 is probably involved in regulating T-cell activation.

Source
Recombinant human CD48 / SLAMF2 Protein (Human CD48, His Tag) Gln 27 - Ser 220 was produced in human 293 cells (HEK293).

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 23.2 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Human CD48, His Tag by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Human 2B4, Fc Tag (Catalog # 2B4-H5255) at 5 μg/mL (100 μL/well) can bind Human CD48, His Tag (Catalog # BC1-H5226) with a linear range of 0.156-2.5 μg/mL.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Korinek V., et al., 1991, Immunogenetics 33:108-112.
(2) Wollscheid B., et al., 2009, Nat. Biotechnol. 27:378-386.