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Recombinant Human GITR / TNFRSF18 Protein, His-Tag, 100µg  

Recombinant Human GITR / TNFRSF18 Protein, His-Tag, 100µg

Recombinant Human GITR / TNFRSF18 Protein, His Tag, HEK293 expressed (rh GITR /TNFRSF18) Gln 26 - Glu 161 was produced in human 293 cells (HEK293)fused with a polyhistidine tag.

Synonym: recombinant, human, protein,GITR,TNFRSF18

More details

GIR-H5228-100

Availability: within 7 days

383,00 €

Background
Glucocorticoid-induced TNFR-related protein (GITR) is also known as Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), activation-inducible TNFR family receptor (AITR), CD antigen CD357, which is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is receptor for TNFSF18, which seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. GITR also mediated NF-kappa-B activation via the TRAF2/NIK pathway.

Source
Recombinant Human GITR, His Tag (GIR-H5228) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Glu 161 (Accession # Q9Y5U5-1).
Predicted N-terminus: Gln 26

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 16.4 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh GITR /TNFRSF18 by the LAL method.

Purity
>92% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Please refer to product data sheet.

References

(1) Gurney A.L., et al., 1999, Curr. Biol. 9:215-218.
(2) Park MS., et al., 2007, Yonsei Med J 48 (5): 839-46.