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Recombinant Cynomolgus / Rhesus macaque B7-1 / CD80 Protein, Fc Tag, 100µg  

Recombinant Cynomolgus / Rhesus macaque B7-1 / CD80 Protein, Fc Tag, 100µg

Recombinant Cynomolgus / Rhesus macaque B7-1, Fc Tag (CD0-C5252) is expressed from human 293 cells (HEK293).

Synonym: Recombinant, cynomolgus, protein, CD80,B7,B7-1,B7.1,BB1,CD28LG

More details

CD0-C5252-100

Availability: within 7 days

312,00 €

Background
B7-1 and B7-2, together with their receptors CD28 and CTLA­4, constitute one of the dominant co-stimulatory pathways that regulate T­ and B­cell responses. Although both CTLA­4 and CD28 can bind to the same ligands, CTLA­4 binds to B7­1 and B7­2 with a 20 ­ 100 fold higher affinity than CD28 and is involved in the down­regulation of the immune response. B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.

Source
Cynomolgus / Rhesus macaque B7-1, Fc Tag (CD0-C5252) is expressed from human 293 cells (HEK293). It contains AA Val 35 - Asn 242 (Accession # G7NXN7). In the region Val 35 - Asn 242, the AA sequence of Cynomolgus and Rhesus macaque B7-1 are homologus.
Predicted N-terminus: Val 35

Molecular Characterization
Cynomolgus B7-1, Fc Tag is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 50.5 kDa. The predicted N-terminus is Val 35. DTT-reduced Protein migrates as 60-90 kDa in SDS-PAGE .

Endotoxin
Less than 1.0 EU per μg of the Cynomolgus B7-1, Fc Tag by the LAL method.

Purity
>95% as determined by reduced SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus CTLA-4, His Tag (Catalog # CT4-C5227) at 2 μg/mL (100 μL/well) can bind Cynomolgus B7-1, Fc Tag (Catalog # CD0-C5252) with a linear range of 1-8 ng/mL.

References

(1) Peach RJ., et al., 1995, J. Biol. Chem. (UNITED STATES) 270 (36): 21181–7.
(2) Stamper CC., et al., 2001, Nature (England) 410 (6828): 608–11.
(3) Bhatia S., et al., 2005, Proc. Natl. Acad. Sci. 102: 15569-15574.
(4) Khan N., et al., 2007, Int. Immunol. 19:477-486.