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Recombinant Human B7-H3 / CD276 Protein, His Tag, 100µg  

Recombinant Human B7-H3 / CD276 Protein, His Tag, 100µg

Recombinant human B7-H3 / CD276 Protein, Leu 29 - Pro 245, produced in human 293 cells (HEK293), His Tag

Synonym: recombinant human B7-H3, CD276, B7 homolog 3

More details

B73-H52E2-100

Availability: within 7 days

348,00 €

Background
B7 homolog 3 (B7-H3), a member of the immunoglobulin superfamily, is also known CD276, which contains two Ig-like C2-type (immunoglobulin-like) domains and two Ig-like V-type (immunoglobulin-like) domains. B7-H3 may participate in the regulation of T-cell-mediated immune response. B7-H3 also plays a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. Furthermore, B7-H3 is involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. It could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy.

Source
Recombinant Human B7-H3, His Tag (B73-H52E2) is expressed from human 293 cells (HEK293). It contains AA Leu 29 - Pro 245 (Accession # Q5ZPR3-2). Predicted N-terminus: Leu 29

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 25.2 kDa. The protein migrates as 40-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

Chapoval A.I., et al., 2001, Nat. Immunol. 2:269-274.
Castriconi R., et al., 2004, Proc. Natl. Acad. Sci. U.S.A. 101:12640-12645.
Wang L., et al., 2005, Eur. J. Immunol. 35:428-438