Recombinant human LIFR /CD118 Protein, Fc Tag, 50µg

Background
Leukemia inhibitory factor receptor also known as LIFR; CD118; FLJ98106; FLJ99923; LIF-R; SJS2; STWS; SWS, is the receptor for leukemia inhibitory factor (LIF). The leukemia inhibitory factor is a polyfunctional cytokine that affects the differentiation, survival, and proliferation of a wide variety of cells in the adult and the embryo. LIF action appears to be mediated through a high-affinity receptor complex composed of a low-affinity LIF binding chain (LIF receptor) and a high-affinity converter subunit, gp130. Both LIFR and gp130 are members of a family of cytokine receptors that includes components of the receptors for the majority of hematopoietic cytokines and for cytokines that affect other systems, including the ciliary neurotrophic factor, growth hormone and prolactin Defects in LIFR are the cause of Stueve-Wiedemann syndrome (SWS), a severe autosomal recessive condition and belongs to the group of the bent-bone dysplasias.[1-5]

Source
Recombinant Human LIF R, Fc Tag (LIR-H4252) is expressed from human 293 cells (HEK293). It contains AA Gln 45 - Ser 833 (Accession # P42702-1). Predicted N-terminus: Gln 45

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 115.5 kDa. The protein migrates as 140-190 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Multifunctional Electrolyte Additive for Bi-electrode Interphase Regulation and Electrolyte Stabilization in Li/LiNi0.8Co0.1Mn0.1O2 Batteries"
Jiang, Xu, Yin et al
ACS Appl Mater Interfaces (2022)
(2) "Ultra-Low Concentration Electrolyte Enabling LiF-Rich SEI and Dense Plating/Stripping Processes for Lithium Metal Batteries"
Chen, You, Li et al
Adv Sci (Weinh) (2022)
(3) "Plasma protein profiling reveals dynamic immunomodulatory changes in multiple sclerosis patients during pregnancy"
Papapavlou Lingehed, Hellberg, Huang et al
Front Immunol (2022) 13, 930947
Showing 1-3 of 700 papers.