Recombinant Human IL1F7b /IL37b Protein, His Tag, 50µg

Background
Interleukin-1 family member 7 (IL1F7b) ,a member of the IL-1 family, is aslo known as interleukin-37, interleukin-1 homolog 4 (IL-1H4), interleukin-1 zeta (IL-1 zeta) and interleukin-1-related protein, which can interact with SMAD3 and bind to IL18R1, but not to IL1R1, with lower affinity than IL18. IL1F7b is a suppressor of innate inflammatory and immune responses involved in curbing excessive inflammation. This function requires SMAD3. Therefore,IL1F7b can suppress, or reduce, proinflammatory cytokine production, including IL1A and IL6, as well as CCL12, CSF1, CSF2, CXCL13, IL1B, IL23A and IL1RN, but spare anti-inflammatory cytokines. Furthermore,IL1F7b can also inhibit dendritic cell activation.

Source
Recombinant Human IL-37, His Tag (ILB-H5141) is expressed from E.coli cells. It contains AA Val 46 - Asp 218 (Accession # AAH20637.1).
Predicted N-terminus: Met

Molecular Characterization
rh IL1F7b / IL37b is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 20.3 kDa. The predicted N-terminus is Met. DTT-reduced Protein migrates as 20.3 kDa in SDS-PAGE.

Endotoxin
Less than 1.0 EU per μg of the rh IL1F7b / IL37b by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).


References
(1) Pan G., et al., 2001, Cytokine 13:1-7.
(2) Sharma S., et al., 2008, J. Immunol. 180:5477-5482.
(3) Nold M.F., et al., 2010, Nat. Immunol. 11:1014-1022.