Recombinant Human IL1F7b /IL37b Protein, His Tag, 50µg

Background
Interleukin-1 family member 7 (IL1F7b) ,a member of the IL-1 family, is aslo known as interleukin-37, interleukin-1 homolog 4 (IL-1H4), interleukin-1 zeta (IL-1 zeta) and interleukin-1-related protein, which can interact with SMAD3 and bind to IL18R1, but not to IL1R1, with lower affinity than IL18. IL1F7b is a suppressor of innate inflammatory and immune responses involved in curbing excessive inflammation. This function requires SMAD3. Therefore,IL1F7b can suppress, or reduce, proinflammatory cytokine production, including IL1A and IL6, as well as CCL12, CSF1, CSF2, CXCL13, IL1B, IL23A and IL1RN, but spare anti-inflammatory cytokines. Furthermore,IL1F7b can also inhibit dendritic cell activation.

Source
Recombinant Human IL-37, His Tag (ILB-H5141) is expressed from E.coli cells. It contains AA Val 46 - Asp 218 (Accession # AAH20637.1).
Predicted N-terminus: Met

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 20.3 kDa. The protein migrates as 21-23 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Elevated Interleukin-37 Associated with Dengue Viral Load in Patients with Dengue Fever"
Zhang, Wang, Zhang et al
Curr Microbiol (2023) 80 (5), 171
(2) "Interleukin-37 ameliorates atherosclerosis by regulating autophagy-mediated endothelial cell apoptosis and inflammation"
Xiao, Song, Zheng et al
Int Immunopharmacol (2023) 118, 110098
(3) "Sex-specific association between adipose tissue inflammation and vascular and metabolic complications of obesity"
Cossins, van den Munckhof, Rutten et al
J Clin Endocrinol Metab (2023)
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