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Recombinant Human VEGF-D Protein, 20µg  

Recombinant Human VEGF-D Protein, 20µg

Recombinant Human VEGF-D Protein, Phe 93 - Ser 201, expressed from human 293 cells (HEK293), His Tag

Synonym: recombinant, human, protein, FIGF,VEGFD

More details

VED-H5228-20

Availability: within 7 days

300,00 €

Background
Vascular endothelial growth factor D (VEGF-D) is also known as C-fos induced growth factor (FIGF), which belongs to the PDGF / VEGF growth factor family and is active in angiogenesis, lymphangiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. This secreted protein VEGF-D / FIGF undergoes a complex proteolytic maturation, generating multiple processed forms that bind and activate VEGFR-2 and VEGFR-3. The structure and function of this protein is similar to those of VEGFC. FIGF / VEGF-D is highly expressed in lung, heart, small intestine and fetal lung. FIGF / VEGF-D may function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR / FLK1) and VEGFR-3 (FLT4) receptors.

Source
Recombinant Human VEGF-D Protein expressed from human 293 cells (HEK293). It contains AA Phe 93 - Ser 201 (Accession # AAH27948).
Predicted N-terminus: Phe 93

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 13.4 kDa. The protein migrates as 19-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Contact us for customized product form or formulation.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

Bioactivity
Please refer to product data sheet.

References

(1) Leppanen V.M., et al., 2011, Blood 117:1507-1515.
(2) Yamada Y., et al., 1997, Genomics 42 (3): 483–8.
(3) Achen MG., et al., 1998, Proc. Natl. Acad. Sci. U.S.A. 95 (2): 548–53.
(4) Yasuoka H., et al., 2005, Mod. Pathol. 18 (8): 1127–33.

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