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Recombinant Human Nectin-3 / CD113 Protein, Fc Tag, 100 µg  

Recombinant Human Nectin-3 / CD113 Protein, Fc Tag, 100 µg

Recombinant Human Nectin-3 / CD113 Protein, Gly 58 - Asp 400, produced in human 293 cells (HEK293), Fc Tag

Synonym: Recombinant human protein,CD113, Nectin-3, PVRL3

More details

PV3-H5255-100

Availability: within 7 days

378,00 €

Background
Poliovirus receptor-related 3 (PVRL3), also known as nectin-3 and CD113, is a human protein of the immunoglobulin superfamily which forms part of adherens junctions. Nectins are immunoglobulin-like adhesion molecules that interact with afadin (AF6; MIM 159559). Afadin is an actin filament-binding protein that connects nectins to the actin cytoskeleton. The nectin-afadin system organizes adherens junctions cooperatively with the cadherin system in epithelial cells. PVRL3 plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Furthermore, PVRL3 induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation.

Source
Recombinant Human Nectin-3, Fc Tag (PV3-H5255) is expressed from human 293 cells (HEK293). It contains AA Gly 58 - Asp 400 (Accession # Q9NQS3).
Predicted N-terminus: Gly 58

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 64.3 kDa. The protein migrates as 80-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Management of Dermatologic Events Associated With the Nectin-4-directed Antibody-Drug Conjugate Enfortumab Vedotin"
Lacouture, Patel, Rosenberg et al
Oncologist (2022) 27 (3), e223-e232
(2) "Nectin cell adhesion molecule 4 regulates angiogenesis through Src signaling and serves as a novel therapeutic target in angiosarcoma"
Tanaka, Murata, Tanegashima et al
Sci Rep (2022) 12 (1), 4031
(3) "Targeting NECTIN-1 Based on CRISPR/Cas9 System Attenuated the Herpes Simplex Virus Infection in Human Corneal Epithelial Cells In Vitro"
Li, Wei, Li et al
Transl Vis Sci Technol (2022) 11 (2), 8
Showing 1-3 of 688 papers.