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Background
Interleukin-12 (IL12) is also known as natural killer cell stimulatory factor (NKSF), cytotoxic lymphocyte maturation factor (CLMF) , is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). IL12 is naturally produced by dendritic cells, macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is involved in the differentiation of naive T cells into Th0 cells and plays an important role in the activities of natural killer cells and T lymphocytes.IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels. Interleukin-12 subunit beta (IL12B) also known as NKSF2, CLMF2 and P40. Interleukin-12 subunit beta has been shown to interact with IL23. A large excess of monomeric IL12B is also secreted by the cells producing IL12, and exhibits no demonstrable biological activity. Overexpression of IL12B gene has been shown to be associated with the pathogenesis of multiple sclerosis. In addition, studies have revealed that the promoter polymorphism of this gene is implicated in the severity of atopic and non-atopic asthma in children.
Source
Recombinant Human IL-12B, His Tag (NK2-H52H7) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (Accession # P29460-1).
Predicted N-terminus: Ile 23
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 36.6 kDa. The protein migrates as 44 kDa and 46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Bioactivity
Please refer to product data sheet.
(1) "Therapeutic targets for gastrointestinal diseases: proteome-wide Mendelian randomization and colocalization analyses"
Zhan, Yang, Li et al
Postgrad Med J (2024)
(2) "Inflammatory plasma protein levels are elevated years before sarcoidosis diagnosis: a nested case-control study in Sweden"
Arkema, Sachs, Dominicus et al
Eur Respir J (2024)
(3) "Implication of IL-12A, IL-12B, IL-6, and TNF single-nucleotide polymorphisms in severity and susceptibility to COVID-19"
Benmansour, Tagajdid, Lahlou et al
Int J Immunopathol Pharmacol (2024) 38, 3946320241279893
Showing 1-3 of 353 papers.
Human IL-12B & IL-12A Heterodimer Protein, His Tag & Flag Tag, 50 µg - 507,00 €
Recombinant Human IL-12B / NKSF2 / p40 Protein, Fc Tag, 20µg - 208,00 €
Recombinant Human IL-12A / NKSF1 / p35 Protein, Fc Tag, 50µg - 325,00 €
Recombinant Biotinylated Human IL-23 alpha & IL-12 beta, Avi Tag (Avitag™), & Tag Free, 25µg - 468,00 €
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