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Recombinant Human IL-12B / NKSF2 / p40 Protein, Fc Tag, 20µg  

Recombinant Human IL-12B / NKSF2 / p40 Protein, Fc Tag, 20µg

Recombinant Human IL12B /P40 Protein (Ile 23 - Ser 328), expressed in human 293 cells (HEK293), Fc Tag

Synonyms: Recombinant human protein, IL12B, CLMF, CLMF2, NKSF, NKSF2, p40

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Availability: within 7 days

208,00 €

Interleukin-12 (IL12) is also known as natural killer cell stimulatory factor (NKSF), cytotoxic lymphocyte maturation factor (CLMF) , is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). IL12 is naturally produced by dendritic cells, macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is involved in the differentiation of naive T cells into Th0 cells and plays an important role in the activities of natural killer cells and T lymphocytes.IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels. Interleukin-12 subunit beta (IL12B) also known as NKSF2, CLMF2 and P40. Interleukin-12 subunit beta has been shown to interact with IL23. A large excess of monomeric IL12B is also secreted by the cells producing IL12, and exhibits no demonstrable biological activity. Overexpression of IL12B gene has been shown to be associated with the pathogenesis of multiple sclerosis. In addition, studies have revealed that the promoter polymorphism of this gene is implicated in the severity of atopic and non-atopic asthma in children.

Recombinant Human IL12B /P40 Protein, Fc Tag (NK2-H5258) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (Accession # AAH67499).
Predicted N-terminus: Ile 23

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 61.3 kDa. The protein migrates as 66-76 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Less than 1.0 EU per μg by the LAL method.

>95% as determined by SDS-PAGE.

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Please refer to product data sheet.

Clinical and Translational Updates

(1) "Asiatic acid and andrographolide reduce hippocampal injury through suppressing neuroinflammation caused by Salmonella typhimurium infection"
Zou, Zhang, Qiu et al
Food Chem Toxicol (2023) 172, 113584
(2) "Retraction Note: Aberrant Methylation of miR-34b and IL-12B mRNA Promoters Contributes to the Reduced Severity of Ankylosing Spondylitis"
Meng, Fan, Li et al
Biochem Genet (2023) 61 (1), 449
(3) "Correlations of psychological distress with plasma cytokine levels and gene mutations in acral and non-acral melanoma"
Kim, Shim, Jung et al
Front Psychiatry (2022) 13, 1024206
Showing 1-3 of 311 papers.