Products

Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Human IL-12B / NKSF2 / p40 Protein, 50 µg  

Recombinant Human IL-12B / NKSF2 / p40 Protein, 50 µg

Recombinant Human IL-12B / NKSF2 / p40 Protein, His Tag (NK2-H52H7) is expressed from human 293 cells (HEK293)

Synonyms: Recombinant human protein, IL12B, CLMF, CLMF2, NKSF, NKSF2, p40

More details

NK2-H52H7-50

Availability: within 7 days

432,00 €

Background
Interleukin-12 (IL12) is also known as natural killer cell stimulatory factor (NKSF), cytotoxic lymphocyte maturation factor (CLMF) , is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). IL12 is naturally produced by dendritic cells, macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is involved in the differentiation of naive T cells into Th0 cells and plays an important role in the activities of natural killer cells and T lymphocytes.IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels. Interleukin-12 subunit beta (IL12B) also known as NKSF2, CLMF2 and P40. Interleukin-12 subunit beta has been shown to interact with IL23. A large excess of monomeric IL12B is also secreted by the cells producing IL12, and exhibits no demonstrable biological activity. Overexpression of IL12B gene has been shown to be associated with the pathogenesis of multiple sclerosis. In addition, studies have revealed that the promoter polymorphism of this gene is implicated in the severity of atopic and non-atopic asthma in children.

Source
Human IL-12B, His Tag (NK2-H52H7) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (Accession # P29460-1).
Predicted N-terminus: Ile 23

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 36.6 kDa. The protein migrates as 44 kDa and 46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh IL12B /P40 by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

Bioactivity
Human IL-12B, His Tag (Cat. No. NK2-H52H7) ELISA bioactivity
Immobilized Anti IL23A & IL12B MAb at 2 μg/mL (100 μL/well) can bind Human IL-12B, His Tag (Cat. No. NK2-H52H7) with a linear range of 0.4-13 ng/mL (QC tested).
Request for protocol

References

(1) Kaliński P, et al., 1997, J. Immunol. 159 (1): 28–35.
(2) Chehimi J, et al., 1993, Eur J Immunol., 23(8):1826-30.
(3) Thierfelder WE, et al., Nature., 382(6587):171-4.
(4) Oppmann B., et al., 2000, Immunity (UNITED STATES) 13 (5): 715–25.