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Recombinant Human BMP-2 Protein, Tag Free, 20 µg  

Recombinant Human BMP-2 Protein, Tag Free, 20 µg

Recombinant Human BMP-2 Protein (Gln 283 - Arg 396), produced in E.coli cells, Tag Free

Synonym: Recombinant Human Protein, BMP2,BMP2A

More details

BM2-H4117-20

Availability: within 7 days

192,00 €

Background
Human bone morphogenetic protein 2 (BMP2) is also known as human BMP-2, BMP-2, BMP2, BMP 2, h-BMP-2, rh-BMP-2, recombinant human BMP-2, recombinant BMP-2, BMP, is a member of the BMP subgroup belonging to the TGF-b superfamily of structurally related signaling proteins. BMP-2 is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with osteoconductive carriers such as collagen and synthetic hydroxyapatite. In addition to its osteogenic activity, BMP-2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. As implied by its name, BMPs promote and regulate bone development, growth, remodeling and repair, in both prenatal development and postnatal growth of eye, heart, kidney, skin, and other tissues. BMP2 has been demonstrated to potently induce osteoblast differentiation in a variety of cell types, and induce apoptosis in human myeloma cell lines as a novel function.

Source
Recombinant Human BMP-2 Protein, Tag Free (Human BMP-2, Tag Free) Gln 283 - Arg 396 (Accession # NP_001191) was produced in E.coli cells at ACROBiosystems.

Molecular Characterization
Human BMP-2, Tag Free contains no "tag", and has a calculated MW of 13 kDa. The predicted N-terminus is Met. DTT-reduced Protein migrates as 13 kDa and non-reduced protein migrates as 24 kDa in SDS-PAGE .

Endotoxin
Less than 1.0 EU per μg of the Human BMP-2, Tag Free by the LAL method.

Purity
>92% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 0.1M acetic acid, pH2.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

Bioactivity-ELISA
Immobilized Human BMP-2, Tag Free (Cat. No. BM2-H4117) at 2 μg/mL (100 μL/well) can bind Human Gremlin, Fc Tag (Cat. No. GR1-H5254) with a linear range of 5-78 ng/mL (QC tested).
 

References

(1) Lai, T.H. et al., (2008) Prostate. 68: 1341-1353.
(2) Kruithof BP,et al. Genesis. 2011, Genesis, 49(11):841-50.
(3) Grgurevic L, et al. Biochem Biophys Res Commun. 2011 Apr 29;408(1):25-31.