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Recombinant Human BMP-2 Protein, Tag Free, 20 µg  

Recombinant Human BMP-2 Protein, Tag Free, 20 µg

Recombinant Human BMP-2 Protein (Gln 283 - Arg 396), produced in E.coli cells, Tag Free

Synonym: Recombinant Human Protein, BMP2,BMP2A

More details

BM2-H4117-20

Availability: within 7 days

192,00 €

Background
Human bone morphogenetic protein 2 (BMP2) is also known as human BMP-2, BMP-2, BMP2, BMP 2, h-BMP-2, rh-BMP-2, recombinant human BMP-2, recombinant BMP-2, BMP, is a member of the BMP subgroup belonging to the TGF-b superfamily of structurally related signaling proteins. BMP-2 is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with osteoconductive carriers such as collagen and synthetic hydroxyapatite. In addition to its osteogenic activity, BMP-2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. As implied by its name, BMPs promote and regulate bone development, growth, remodeling and repair, in both prenatal development and postnatal growth of eye, heart, kidney, skin, and other tissues. BMP2 has been demonstrated to potently induce osteoblast differentiation in a variety of cell types, and induce apoptosis in human myeloma cell lines as a novel function.

Source
Recombinant Human BMP-2 Protein, Tag Free (BM2-H4117) is expressed from E.coli cells. It contains AA Ala 284 - Arg 396 (Accession # NP_001191).
Predicted N-terminus: Met

Molecular Characterization
This protein carries no "tag".
The protein has a calculated MW of 12.8 kDa. the protein migrates as 14 kDa under reducing (R) condition, and 28 kDa under non-reducing (NR) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg of the Human BMP-2, Tag Free by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 100 mM Acetic Acid, pH2.8. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

References

(1) Extraction and characterization of matrix protein from pacific oyster (Crassostrea gigs) shell and its anti-osteoporosis properties in vitro and in vivo
Feng, Jiang, Zhang et al
Food Funct (2021)
(2) Cyclic Tensile Stress to Rat Thoracolumbar Ligamentum Flavum Inducing the Ossification of Ligamentum Flavum: An In Vivo Experimental Study
Zhao, Yuan, Cheng et al
Spine (Phila Pa 1976) (2021) 46 (17), 1129-1138
(3) Ba/Mg co-doped hydroxyapatite/PLGA composites enhance X-ray imaging and bone defect regeneration
Liu, Ma, Chen et al
J Mater Chem B (2021)
Showing 1-3 of 11685 papers.
Please refer to product data sheet.