Recombinant Human BMP-2 Protein, premium grade, 2x 100 µg

Background
Human bone morphogenetic protein 2 (BMP2) is also known as human BMP-2, BMP-2, BMP2, BMP 2, h-BMP-2, rh-BMP-2, recombinant human BMP-2, recombinant BMP-2, BMP, is a member of the BMP subgroup belonging to the TGF-b superfamily of structurally related signaling proteins. BMP-2 is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with osteoconductive carriers such as collagen and synthetic hydroxyapatite. In addition to its osteogenic activity, BMP-2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. As implied by its name, BMPs promote and regulate bone development, growth, remodeling and repair, in both prenatal development and postnatal growth of eye, heart, kidney, skin, and other tissues. BMP2 has been demonstrated to potently induce osteoblast differentiation in a variety of cell types, and induce apoptosis in human myeloma cell lines as a novel function.

Source
Recombinant Human BMP-2 Protein, premium grade(BM2-H4117) is expressed from E. coli cells. It contains AA Ala 284 - Arg 396 (Accession # NP_001191).
Predicted N-terminus: Met
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.

Molecular Characterization
This protein carries no "tag".
The protein has a calculated MW of 12.8 kDa. the protein migrates as 14 kDa under reducing (R) condition, and 28 kDa under non-reducing (NR) condition (SDS-PAGE).

Endotoxin
Less than 0.2 EU per μg by the LAL method.

Sterility
The sterility testing was performed by membrane filtration method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 100 mM Acetic Acid, pH2.8 with trehalose as protectant.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "bmp-2 Gene-Transferred Skeletal Muscles with Needle-Type Electrodes as Efficient and Reliable Biomaterials for Bone Regeneration"
Kawai, Yoshida, Kato et al
Materials (Basel) (2024) 17 (4)
(2) "Statement of Retraction: Exosome-delivered BMP-2 and polyaspartic acid promotes tendon bone healing in rotator cuff tear via Smad/RUNX2 signaling pathway"
Bioengineered (2024) 15 (1), 2299604
(3) "Tendon stem cells seeded on dynamic chondroitin sulfate and chitosan hydrogel scaffold with BMP2 enhance tendon-to-bone healing"
Zhang, Wen, Liao et al
Heliyon (2024) 10 (4), e25206
Showing 1-3 of 12965 papers.