Recombinant Human IL-37 / IL-1F7 Protein, His Tag, 50µg

Background
Interleukin-1 family member 7 (IL1F7b) ,a member of the IL-1 family, is aslo known as interleukin-37, interleukin-1 homolog 4 (IL-1H4), interleukin-1 zeta (IL-1 zeta) and interleukin-1-related protein, which can interact with SMAD3 and bind to IL18R1, but not to IL1R1, with lower affinity than IL18. IL1F7b is a suppressor of innate inflammatory and immune responses involved in curbing excessive inflammation. This function requires SMAD3. Therefore,IL1F7b can suppress, or reduce, proinflammatory cytokine production, including IL1A and IL6, as well as CCL12, CSF1, CSF2, CXCL13, IL1B, IL23A and IL1RN, but spare anti-inflammatory cytokines. Furthermore,IL1F7b can also inhibit dendritic cell activation.

Source
Recombinant Human IL-37, His Tag (ILB-H51H5) is expressed from E.coli cells. It contains AA Lys 27 - Asp 192 (Accession # Q9NZH6-2).

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 20.1 kDa. The protein migrates as 20-23 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 1 mM TCEP, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "IL-37 alleviates TNF-α-induced pyroptosis of rheumatoid arthritis fibroblast-like synoviocytes by inhibiting the NF-κB/GSDMD signaling pathway"
Ren, Chen, Che et al
Immunobiology (2023) 228 (3), 152382
(2) "IL-37 Ameliorates Renal Fibrosis by Restoring CPT1A-Mediated Fatty Acid Oxidation in Diabetic Kidney Disease"
Xiong, He, Liu et al
Kidney Dis (Basel) (2023) 9 (2), 104-117
(3) "Relative CTLA-4, PTPN-22, and interleukin 37 mRNA expressions in patients with lupus nephritis"
A Moneim, Hosny, Omar et al
Reumatol Clin (Engl Ed) (2023) 19 (4), 180-187
Showing 1-3 of 643 papers.