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Recombinant Human Fc epsilon RI alpha Protein, His tag, 100 µg  

Recombinant Human Fc epsilon RI alpha Protein, His tag, 100 µg

Recombinant Human Fc epsilon RI alpha Protein (Val 26 - Gln 205), produced in human 293 cells (HEK293), His tag (MALS verified)

Synonym
Recombinant human protein, FCER1A,FCE1A,FcERI

More details

FCA-H5228-100

Availability: within 7 days

300,00 €

Background
High affinity immunoglobulin epsilon receptor subunit alpha (FCER1A) is also known as Fc-epsilon RI-alpha (FcERI), IgE Fc receptor subunit alpha, FCE1A. FCER1A contains two Ig-like (immunoglobulin-like) domains. FCER1A binds to the Fc region of immunoglobulins epsilon and is a high affinity receptor. FCER1A is responsible for initiating the allergic response, which binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines. FCER1A plays a central role in allergic disease, coupling allergen and mast cell to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma.

Source
Recombinant Human Fc epsilon RI alpha, His Tag (FCA-H5228) is expressed from human 293 cells (HEK293). It contains AA Val 26 - Gln 205 (Accession # P12319-1).
Predicted N-terminus: Val 26

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 22.9 kDa. The protein migrates as 37-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 12 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "The Acari Hypothesis, II: Interspecies Operability of Pattern Recognition Receptors"
Retzinger, Retzinger
Pathogens (2021) 10 (9)
(2) "Antagonism of Protease Activated Receptor-2 by GB88 Reduces Inflammation Triggered by Protease Allergen Tyr-p3"
Wang, Yu, Tsai et al
Front Immunol (2021) 12, 557433
(3) "A novel detection method for cross-linking of IgE-receptors by autoantibodies in chronic spontaneous urticaria"
Koga, Yokooji, Ogino et al
Allergol Int (2021)
Showing 1-3 of 1718 papers.