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Recombinant Human Fc epsilon RI alpha Protein, His tag, 100 µg  

Recombinant Human Fc epsilon RI alpha Protein, His tag, 100 µg

Recombinant Human Fc epsilon RI alpha Protein (Val 26 - Gln 205), produced in human 293 cells (HEK293), His tag

Synonym
Recombinant human protein, FCER1A,FCE1A,FcERI

More details

FCA-H5228-100

Availability: within 7 days

300,00 €

Background
High affinity immunoglobulin epsilon receptor subunit alpha (FCER1A) is also known as Fc-epsilon RI-alpha (FcERI), IgE Fc receptor subunit alpha, FCE1A. FCER1A contains two Ig-like (immunoglobulin-like) domains. FCER1A binds to the Fc region of immunoglobulins epsilon and is a high affinity receptor. FCER1A is responsible for initiating the allergic response, which binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines. FCER1A plays a central role in allergic disease, coupling allergen and mast cell to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma.

Source
Human Fc epsilon RI alpha, His Tag (FCA-H5228) is expressed from human 293 cells (HEK293). It contains AA Val 26 - Gln 205 (Accession # P12319-1).
Predicted N-terminus: Val 26

Molecular Characterization
Human Fc epsilon RI alpha, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 21.9 kDa. The predicted N-terminus is Val 26. The reducing (R) protein migrates as 40-60 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

Bioactivity-ELISA
Please refer to product data sheet.

References

(1) Yagi S., et al., 1994, Eur. J. Biochem. 220:593-598.
(2) Garman S.C., et al., 1998, Cell 95:951-961.
(3) Garman S.C., et al., 2001, J. Mol. Biol. 311:1049-1062.