Recombinant Human IL-18 Protein, Tag Free (MALS verified), 20µg

Background
Interleukin-18 (IL-18) is a potent proinflammatory cytokine that induces interferon-gamma (IFN-gamma) production from Th1 cells, NK cells and activated macrophages, particularly in the presence of IL-12. IL-18 also functions in developmental regulation of T-lymphocyte helper type I cells and in Fas-mediated cytotoxicity. Suppression of IL-18 activity is being investigated for treatment of chronic inflammatory diseases such as Crohn's disease and rheumatoid arthritis. It acts by inducing heterodimerization of the two subunits of its receptor, IL-18RAlpha and IL-18RBeta shows structural similarity to IL-1.

Source
Recombinant Human IL-18, Tag Free (IL8-H5113) is expressed from E.coli cells. It contains AA Tyr 37 - Asp 193 (Accession # Q14116-1).
Predicted N-terminus: Met

Molecular Characterization
This protein carries no "tag".
The protein has a calculated MW of 18.3 kDa. The protein migrates as 18 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Inhibition of GSK-3β/HIF-1α pathway alleviates NLRP3-mediated pyroptosis induced by high glucose in renal tubular epithelial cells"
Wan, Jiang, Liu et al
Exp Physiol (2022)
(2) "Myeloid autophagy genes protect mice against fatal TNF- and LPS-induced cytokine storm syndromes"
Wang, Sansone, Smirnov et al
Autophagy (2022)
(3) "Apigenin and apigenin-7, 4'-O-dioctanoate protect against acrolein-aggravated inflammation via inhibiting the activation of NLRP3 inflammasome and HMGB1/MYD88/NF-κB signaling pathway in Human umbilical vein endothelial cells (HUVEC)"
Yu, Jiang, Liu et al
Food Chem Toxicol (2022)
Showing 1-3 of 14294 papers.