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Recombinant Biotinylated Human IL-11 Protein, His,Avitag™ (MALS verified), 25µg  

Recombinant Biotinylated Human IL-11 Protein, His,Avitag™ (MALS verified), 25µg

Recombinant Biotinylated Human IL-11 Protein, AA Pro 22 - Leu 199, expressed from human 293 cells (HEK293), His,Avitag™ (MALS verified)

Synonyms: Recombinant, IL-11, Interleukin-11, AGIF, Oprelvekin, IL11

More details

IL1-H82E3-25

Availability: within 7 days

455,00 €

Background
Interleukin-11 (IL-11) is a pleiotropic cytokine that stimulates megakaryocytopoiesis, resulting in increased production of platelets, as well as activating osteoclasts, inhibiting epithelial cell proliferation and apoptosis, and inhibiting macrophage mediator production. These functions may be particularly important in mediating the hematopoietic, osseous and mucosal protective effects of IL-11. The cytokine also possesses anti-inflammatory activity, and has been proposed as a therapeutic agent in the treatment of chronic inflammatory diseases, such as Crohn's disease and rheumatoid arthritis. Although IL-11 was initially believed to be restricted to mammals, subsequent studies demonstrated it to be expressed in fish. Despite close similarity in gene structure and conservation of key amino acids between fish and mammalian IL-11, they share relatively low overall amino acid identity and may not necessarily be functionally analogous.

Source
Recombinant Biotinylated Human IL-11, His,Avitag (IL1-H82E3) is expressed from human 293 cells (HEK293). It contains AA Pro 22 - Leu 199 (Accession # P20809-1).
Predicted N-terminus: His

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 22.7 kDa. The protein migrates as 25-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Biotin:Protein Ratio
Passed as determined by the HABA assay / binding ELISA.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 0.5 M Arginine, pH7.4 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "TRPV4 differentially controls inflammatory cytokine networks during static and dynamic compression of the intervertebral disc"
Easson, Savadipour, Gonzalez et al
JOR Spine (2023) 6 (4), e1282
(2) "Molecular Determinants of Uterine Receptivity: Comparison of Successful Implantation, Recurrent Miscarriage, and Recurrent Implantation Failure"
Günther, Allahqoli, Deenadayal-Mettler et al
Int J Mol Sci (2023) 24 (24)
(3) "The Human GP130 Cytokine Receptor and Its Expression-an Atlas and Functional Taxonomy of Genetic Variants"
Chen, van Zon, Adams et al
J Clin Immunol (2023) 44 (1), 30
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